实时荧光PCR法快速分离食源性致病菌

目的建立快速的食源性沙门菌和志贺菌检测方法,以提高对该菌的检验速度和准确性,为公共卫生,食源性致病菌监测,突发性群体性食物中毒事件提供技术支持。方法采用实时荧光PCR法和培养法对40份不同类食品进行沙门菌和志贺菌的检测,并对分离的菌株进行血清分型和定量检测,比较两种方法的特异性,灵敏度,准确性。结果采用实时荧光PCR法检测40份样本,其中8份沙门菌核酸阳性,阳性率20%;采用培养法检测这40份样本,4份样品分离出沙门菌,阳性率10%,且4份样品经核酸检测均为阳性,32份核酸阴性样本采用培养法检测,均未分离到沙门菌和志贺菌。结论实时荧光PCR法结合传统分离培养法适宜食品中沙门菌,志贺菌的快速筛查鉴定及食物中毒等突发事件的快速诊断。

Rapid Isolation of Food-borne Pathogenic Bacteria by Real-time PCR

Objective To provide technical support for public health,food-borne pathogenic bacteria mornitoring and sudden mass unexpected food poisonous accidents by establishing rapid methods of food-borne Salmonella and Shigella detection.Methods Forty food samples were conducted salmonella and shigella detection with cultiation and real-time PCR methods,with the isolated strains were conducted serological and quantitative detection.Compared the specifity,sensitivity and accuracy of two methods.Results Forty samples were detected by real-time PCR,in which 8 were Salmonella nucleic acid positive,with the positive rate was 20%;40 samples were detected by cultivation method,in which Salmonella were isolated from 4 samples,with positive rate was 10%,and all the 4 samples were nucleic acid possitive.Cultivation method was conducted on the 32 salmonella negative samples,and no Salmonella and Shigella was isolated.Conclusion Real-time PCR combined with traditional culture methods is suitable for food Salmonella,Shigella identification and rapid screening of food poisoning and other emergencies.