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| 病毒分离、RT-PCR和实时荧光RT-PCR法检测肠道病毒71型比较 | |
| 引用本文： | 曹春远,陈前进,温联煌,王秉楠,何云,廖亦红,李新文.病毒分离、RT-PCR和实时荧光RT-PCR法检测肠道病毒71型比较[J].预防医学情报杂志,2011,27(9):742-744. |
| 作者姓名： | 曹春远 陈前进 温联煌 王秉楠 何云 廖亦红 李新文 |
| 作者单位： | 1. 龙岩市疾病预防控制中心,福建龙岩,364000 2. 龙岩市疾病预防控制中心，福建龙岩364000;福建医科大学龙岩市疾病预防控制中心教学基地 3. 福建医科大学公共卫生学院 |
| 摘 要： | 评价病毒分离培养、RT-PCR及实时荧光RT-PCR法对于肠道病毒7l型的检测效果。方法分别用疖毒分离培养、RT-PCR、实时荧光RT-PCR方法对2009年龙岩市手足口病哨点监测医院送检的153份手足口病患者咽捌子样本进行EV71检测，并对检测结果进行配对卡方检验。结果3种方法总体符合率为81．0％，3种检测方法对样本呻EV71阳性检出率差异无统计学意义（P〉0．05）。结论病毒分离、RT-PCR和实时荧光RT-PCR检测方法均适用于EV71的检测，可以作为EV71的日常临床诊断方法。 |
| 关 键 词： | 手足口病 EV71 病毒分离 RT-PCR 实时荧光RT-PCR |
Comparison of Virus Isolation, RT- PCR and Real- time RT- PCR Detections of Enterovirus 71 |
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| CAO Chun-yuan,CHEN Qian-jin,WEN Lian-huang,et al..Comparison of Virus Isolation, RT- PCR and Real- time RT- PCR Detections of Enterovirus 71[J].Journal of Preventive Medicine Information,2011,27(9):742-744. | |
| Authors: | CAO Chun-yuan CHEN Qian-jin WEN Lian-huang |
| Institution: | CAO Chun-yuan1,CHEN Qian-jin1,2,WEN Lian-huang3,et al. 1Longyan Municipal Center for Disease Control and Prevention,Longyan 364000,Fujian Province,China. 2Teaching Base for Longyan Municipal Center for Disease Control and Prevention,Fujian Medical University,China. 3School of Public Health,China. |
| Abstract: | Objective To evaluate the effect of three detection methods which applied to detect enterovirus 71(EV71),including virus isolation,RT-PCR and real-time RT-PCR.Methods Totally 153 cases' throat swab samples which submitted from Longyan City's Hand-Foot and Mouth Disease(HFMD) surveillance hospitals in 2009,were conducted virus isolation,RT-PCR and real-time RT-PCR to detect EV71.Results The general consistency rate of 3 methods was 81.0%,without statistical difference among the positive rates of these 3 meth... |
| Keywords: | hand foot and mouth disease(HFMD) EV71 virus isolation RT-PCR real-time RT-PCR |
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