L-精氨酸对脂多糖诱导免疫应激大鼠血清生化指标和肝脏的影响

目的探讨外源性精氨酸对动物机体在免疫应激状态下的保护作用。方法以腹腔注射大肠杆菌内毒素(LPS)的方式复制免疫应激动物模型。将SD大鼠32只随机分成4组:正常对照组;生理盐水配对组;免疫应激组(LPS组);L-精氨酸+LPS组(L-Arg+LPS组)。结果腹腔注射LPS 4mg/kg.bw,可以使大鼠产生免疫应激。L-Arg+LPS组大鼠血清中Arg浓度非常显著高于其余各组(P<0.01),与对照组相比,L-Arg+LPS组和LPS组血液中谷丙转氨酶(ALT)活性非常显著(P<0.01),L-Arg+LPS组和LPS组血清中总的一氧化氮合酶(T-NOS)活性显著增高(P<0.05),NO浓度和诱导型一氧化氮合酶(iNOS)活性达到非常显著(P<0.01)。免疫组化显示:L-Arg+LPS组的大鼠肝组织内皮型一氧化氮合酶(eNOS)表达呈强阳性,其他组未见eNOS表达;而LPS组肝组织iNOS表达呈中度阳性,L-Arg+LPS组iNOS表达呈痕迹反应,对照组和配对组均无iNOS表达。L-Arg+LPS组和LPS组分别与对照组相比,肝细胞凋亡极显著增多(P<0.01),但L-Arg+LPS组细胞凋亡数显著低于LPS组(P<0.05),对照组和配对组之间差异不显著。结论对免疫应激大鼠添加精氨酸后,可抑制由iNOS催化生成NO而导致的肝脏组织炎症及肝细胞凋亡,缓减免疫应激对大鼠机体的损害作用。

EFFECT OF L-ARGININE ON SERUM BIOCHEMICAL INDICES AND LIVER OF RATS UNDER IMMUNOLGICAL STRESS INDUCDED BY LIPOPOLYSACCHAIDES

Objective To investigate the protective role of exogenous L-arginine on rats under immunological stress.Method Animal model of immunological stress was induced by Escherichia coli endotoxin(LPS) with intraperitoneal injection.Thirty-two rats were randomly divided into 4 groups:normal control group,saline paired group,immunological stress group(LPS group) and L-Arginine+LPS group(L-Arg+LPS).Results Immunological stress was induced by intraperitoneal injection LPS 4 mg/kg·bw in rats.Serum arginine concentrations of rats in L-Arg+LPS group were extremely significantly higher than that of the other groups(P<0.01).The activities of ALT in LPS and L-Arg+LPS group were extremely significantly higher than the control group(P<0.01).Compared with the control group,the activities of T-NOS in LPS and L-Arg+LPS group were significantly increased(P<0.05),and the concentrations of NO and the activities of iNOS extremely significantly increased(P<0.01).The results of immunohistochemistry showed that eNOS expression of liver in L-Arg+LPS group was extremely positive,while the remaining groups had no eNOS expression.iNOS expression in liver was moderately positive in LPS group,and slightly positive in L-Arg+LPS group.There was no iNOS expression in control and paired group.Compared with control group,apoptosis of liver cells in LPS and L-Arg+LPS group was extremely significantly increased(P<0.01),and apoptosis of liver cells in L-Arg+LPS group was significantly lower than that of LPS group(P<0.05).There was no significant difference between control and saline paired group(P>0.05).Conclusion Supplementation with arginine for rats under immunological stress could inhibit the inflammation and apoptosis of liver cells caused by NO from iNOS catalytic generation,and reduced the damage of immunological stress.