羊胡髭草抗炎利尿作用的实验研究

目的：研究羊胡髭草的抗炎利尿作用，并进行相关急性毒性实验。方法：小鼠随机分为5组：生理盐水组、阿司匹林组（0.05 g·kg-1）、羊胡髭草水提液高、中、低剂量组10、5、2.5 g（生药）·kg-1]，灌胃给药，每天1 次，连续灌胃3 天后，采用二甲苯致小鼠耳廓肿胀法造模，计算耳廓肿胀度及肿胀抑制率；另取大鼠随机分为5 组：生理盐水组、阿司匹林组（0.05 g·kg-1）、羊胡髭草水提液高、中、低剂量组10、5、2.5 g（生药）·kg-1]，灌胃给药，每天1 次，连续灌胃5 天后，采用角叉菜胶致大鼠足趾肿胀法造模，计算足趾肿胀率；另取大鼠随机分为5 组：生理盐水组、呋塞米片组（0.01 g·kg-1）、羊胡髭草水提液高、中、低剂量组10、5、2.5 g（生药）·kg-1]，灌胃给药，每天1次，连续5天，采用水负荷方法造模，计算5 h内尿量；采用最大耐受量（MTD）测定其急性毒性。结果：羊胡髭草水提液高、中、低3个剂量组对小鼠耳廓肿胀度、肿胀抑制率、大鼠足趾肿胀率均有明显的抑制作用，对大鼠5 h内尿量有明显增加作用；小鼠羊胡髭草水提液灌胃给药MTD为640 g（生药）·kg-1。结论：羊胡髭草对实验动物模型有明显的抗炎利尿作用，且急性毒性很小。

Experimental Study on Anti-inflammatory, Diuretic Effect and Acute Toxicity of Herb of Lanceolate Sedge

This study was aimed to investigate the anti-inflammatory, diuretic effect and acute toxicity of Herb of Lanceolate Sedge. The mice were randomly divided into 5 groups, which were the physiological saline group, aspirin group (0.05 g?kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was given to mice once a day for 3 days. Auricular swelling induced by dimethyl benzene was used in the model establishment to calculate the swollen ear degree and the swollen inhibiting rate. And rats were randomly divided into 5 groups, which were the physiological saline group, aspirin group (0.05 g?kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was given to rats once a day for 5 days. Carrageenan induced paw edema was used in the model establishment to calculate the paw swollen rate. And rats were randomly divided into 5 groups, which were the physiological saline group, Furosemide tablets group (0.01 g?kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge(10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was given to rats once a day for 5 days. Water-loaded was used in the model establishment to calculate the 5-hour urine amount. The maximum tolerance dose (MTD) was used in the determination of the acute toxicity. The results showed that the three groups of HD, MD and LD of water extract of Herb of Lanceolate Sedge had obvious inhibiting effects on the swollen ear degree and the swollen inhibiting rate among mice, and the paw swollen rate of rats. It had significant increasing effect on the 5-hour urine amount. The MTD of intragastric administration of water extract of Herb of Lanceolate Sedge was 640 g crude drug per kg for mice. It was concluded that Herb of Lanceolate Sedge displayed obvious effects on anti-inflammatory and dieresis with low acute toxicity.