参附注射液对大鼠局灶性脑缺血再灌注损伤的保护作用及其机制研究

目的]探讨参附注射液对局灶性脑缺血大鼠Na-K-Cl协同转运蛋白1(NKCC1)的影响。方法]雄性SD大鼠随机分为假手术组(Sham),缺血模型组(Ischemia)和缺血后给予参附注射液治疗组(SFI)。利用线栓法制作脑缺血/再灌注模型,再灌注3 h及24 h后进行神经功能评分,再灌注24 h后2,3,5氯化三苯基四氮唑蓝(TTC)法比较脑梗死面积,免疫组织化学及蛋白印迹法检测NKCC1的表达。结果]参附注射液治疗组的脑梗死面积、神经功能缺损程度均比对照组明显降低(P0.05)。和对照组相比,SFI组的NKCC1表达明显低于Ischemia组(P0.05)。结论]参附注射液对大鼠脑缺血模型的神经保护作用可能与抑制NKCC1相关。

Neuroprotective role and its mechanism of Shenfu injection on focal ischemic stroke in rats

Objective] To investigate the effect of Shenfu injection on the expression of Na-K-Cl-cotransporter 1 (NKCC1) in rats with focal cerebral ischemic stroke. Methods] The ischemic stroke was generated by 90 min occlusion of right middle cerebral artery. After 24 h reperfusion, neurological score was evaluated. The cerebral infarct volumes were quantified by 2, 3, 4-triphenytetrazolium-chloride (TTC), and the NKCC1expression were evaluated by Western blot and immunofluorescence methods. Results] Shenfu injection-treatment group (SFI) significantly reduced the infarct volume (P<0.05) and the degree of neurological deficits (P<0.05). Compared to the ischemia group, the protein expression of NKCC1 was significantly reduced in the SFI group. Conclusion] Shenfu injection plays a neuroprotective role in rats with experimental cerebral ischemia, which was associated with the inhibition of NKCC1.