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内皮祖细胞移植对糖尿病兔下肢缺血的病理及血管内皮生长因子的影响
引用本文:于萍,李强,刘颖,李静宜,王薇,孙玉倩,张巾超. 内皮祖细胞移植对糖尿病兔下肢缺血的病理及血管内皮生长因子的影响[J]. 中国实用内科杂志, 2007, 27(15): 1207-1209
作者姓名:于萍  李强  刘颖  李静宜  王薇  孙玉倩  张巾超
作者单位:哈尔滨医科大学附属第二医院内分泌科,黑龙江,哈尔滨,150086
摘    要:目的探讨内皮祖细胞(EPC)移植治疗糖尿病兔下肢缺血的病理及缺血肌肉中血管内皮生长因子(VEGF)的变化。方法2006年1月至9月在哈尔滨医科大学附属第二医院内分泌科将30只健康日本大耳白兔随机分为3组,即糖尿病磷酸盐缓冲液(PBS)对照组(A组)8只、糖尿病内皮祖细胞移植治疗组(B组)14只、正常血糖内皮祖细胞移植治疗组(C组)8只。骨髓来源的内皮祖细胞经体外扩增培养7d后,通过肌内注射进行细胞移植,用病理改变及肌浆中VEGF的变化评价治疗效果。结果EPC移植后14d,病理示3组毛细血管密度分别为(9.29±1.63)个/视野、(12.60±2.16)个/视野、(12.51±1.56)个/视野;血管数/肌束数分别为0.66±0.05,0.83±0.11,0.90±0.13;肌浆中VEGF质量分数[每克肌肉中VEGF因子的质量(ng)]分别为0.22±0.05,0.30±0.07,0.31±0.08;糖尿病内皮祖细胞移植治疗组与糖尿病PBS对照组比较,毛细血管密度、血管数/肌束数、VEGF值差异均有显著性意义,P<0.05。结论EPC移植能有效治疗糖尿病兔下肢缺血。

关 键 词:内皮祖细胞  移植  糖尿病兔模型  下肢缺血
文章编号:1005-2194(2007)15-1207-03
修稿时间:2007-02-17

Pathologic and VEGF changes of transplantation of endothelial progenitor cells for hindlimb ischemia in diabetic rabbit model
YU Ping,LI Qiang,LIU Ying,et al.. Pathologic and VEGF changes of transplantation of endothelial progenitor cells for hindlimb ischemia in diabetic rabbit model[J]. Chinese Journal of Practical Internal Medicine, 2007, 27(15): 1207-1209
Authors:YU Ping  LI Qiang  LIU Ying  et al.
Affiliation:YU Ping,LI Qiang,LIU Ying,et al.Department of Endocrinology,the Second Hospital Affiliated to Harbin Medical University,Harbin 150086,China
Abstract:Objective To explore the effects of transplantation of endothelial progenitor cells(EPC)from autologous bone marrow for hindlimb ischemia in diabetic rabbit model,and the difference between diabetes and nondiabetes.Methods Rabbits were randomly allocated into three groups for transplantation therapy,PBS control group(n=8,A group),EPC transplantation with diabetes group(n=14,B group),EPC transplantation without diabetes group(n=8,C group).Diabetic rabbit model was established by injecting alloxan,and hindlimb ischemia was induced by complete excision of femoral artery.Diabetic rabbits with hindlimb ischemia were therapied by transplanted ex vivo expanded EPC,and then effects were assessed by capillary density and capillary to muscle fiber ratio,and VEGF in muscle was also determined.Results Capillary density,capillary to muscle fiber ratio,and VEGF in muscle increased significantly in EPC transplantation group with or without diabetes after transplanting 14 days(P<0.05).Conclusion Endothelial progenitor cells transplantation is an effective therapeutic method for hindlimb ischemia in diabetic rabbit model.
Keywords:EPC  Transplantation  Diabetic rabbit model  Hindlimb ischemia
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