IS element IS<Emphasis Type="Italic">16</Emphasis> as a molecular screening tool to identify hospital-associated strains of <Emphasis Type="Italic">Enterococcus faecium</Emphasis> |
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Authors: | Guido Werner Carola Fleige Uta Geringer Willem van Schaik Ingo Klare Wolfgang Witte |
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Institution: | (1) Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany;(2) University Medical Centre, Utrecht, The Netherlands |
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Abstract: | Background Hospital strains of Enterococcus faecium could be characterized and typed by various molecular methods (MLST, AFLP, MLVA) and allocated to a distinct clonal complex
known as MLST CC17. However, these techniques are laborious, time-consuming and cost-intensive. Our aim was to identify hospital
E. faecium strains and differentiate them from colonizing and animal variants by a simple, inexpensive and reliable PCR-based screening
assay. We describe here performance and predictive value of a single PCR detecting the insertion element, IS16, to identify hospital E. faecium isolates within a collection of 260 strains of hospital, animal and human commensal origins. |
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