Antibody responses to Rhoptry-Associated Protein-1 (RAP-1) of Plasmodium falciparum parasites in humans from areas of different malaria endemicity |
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| Authors: | PH JAKOBSEN JAL KURTZHALS EM RILEY L HVIID TG THEANDER S MORRIS-JONES JB JENSEN RAL BAYOUMI RG RIDLEY & BM GREENWOOD |
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| Institution: | Centre for Medical Parasitology, Department of Infectious Diseases, Department of Clinical Microbiology, Copenhagen University Hospital (Rigshospitalet) Tagensvej 20, DK 2200, Copenhagen N, and Institute for Medical Microbiology and Immunology, University of Copenhagen, Denmark,; Medical Research Council Laboratories, Fajara, The Gambia,; Department of Microbiology, Brigham Young University, Provo, UT, USA,; Department of Biochemistry, University of Khartoum, Sudan,; Pharma Division, Preclinical Research, F.Hoffmann-La Roche Ltd., Basel Switzerland |
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| Abstract: | Plasma IgM and IgG antibody reactivities against the recombinant Plasmodium falciparum protein, Rhoptry Associated Protein-1 (rRAP-1) were measured by ELISA in individuals from Sudan, Indonesia, Kenya and The Gambia living in areas of different malaria endemicity. IgG and IgM reactivities to rRAP-1 increased with malaria endemicity. IgG reactivities were associated with spleen rates in Indonesia with high malaria endemicity while IgM reactivities were associated with spleen rates in Kenya with low malaria endemicity. IgG and IgM reactivities to rRAP-1 increased during acute episodes of P. falciparum malaria in Sudanese adults and IgG reactivities remained high one month after treatment in all adults tested. Antibody reactivities to rRAP-1 in Gambian children in the dry season were higher in children with parasitaemia than in children without detectable parasitaemia. Antibody reactivities were not associated with protection against clinical episodes in the following rainy season but higher antibody reactivities were detectable at the end of the rainy season. There was no difference in antibody reactivity to rRAP-1 between Gambian children with mild or severe malaria. |
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| Keywords: | malaria antibodies Rhoptry-associated protein-1 |
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