江苏地区无偿献血人群巴贝虫感染调查

目的　了解江苏地区无偿献血人群巴贝虫感染情况，为输血安全提供科学依据。方法　2017年2–5月对江苏省血液中心采集的950人份无偿献血者血样，以巴贝虫分泌抗原（BmSA1）为诊断靶标分子，采用双抗原夹心酶联免疫吸附试验（ELISA）检测单份血清标本靶向巴贝虫特异性总抗体水平，对抗体阳性样品制作血涂片进行镜检，并提取DNA进行巢式PCR扩增确认寄生虫血症；分析不同性别、年龄和职业献血者巴贝虫抗体阳性率。结果　江苏地区950人份无偿献血人群巴贝虫抗?BmSA1抗体阳性率为0.53%，5例巴贝虫抗?BmSA1抗体阳性血样镜检和巢式PCR结果均为阴性。不同性别（χ2] = 0.01，P = 0.92）和年龄（χ2] = 0.11，P = 0.95）献血者巴贝虫抗?BmSA1抗体阳性率间差异均无统计学意义，但不同职业献血者巴贝虫抗?BmSA1抗体阳性率间差异具有统计学意义（χ2] = 11.93，P < 0.05）。结论　江苏地区无偿献血者中有巴贝虫感染者，应予以重视。

Sero-prevalence of Babesia infection among voluntary blood donors in Jiangsu Province

Objective　To evaluate the sero?positivity of Babesia infection in voluntary blood donors in Jiangsu region, so as to provide the evidence for transfusion safety. Methods　A total of 950 blood samples were collected from voluntary blood donors in Jiangsu Provincial Blood Center from February to May, 2017, and detected by double antigen sandwich ELISA targeting peptides derived from B. microti-secreted antigen 1 (BmSA1). The positive samples were confirmed by microscopy and nested?PCR to determine parasitemia. The prevalence of anti?BmSA1 was analyzed between/among different genders, ages and occupations of the blood donors. Results　Of the 950 blood screened samples, 5 were positive for anti?BmSA1, and the sero?prevalence of Babesia infection was 0.53%. The 5 samples were all negative by microscopy and nested?PCR. There were no gender? (χ2] = 0.01, P = 0.92) or age?specific differences (χ2] = 0.11, P = 0.95) in the sero?prevalence of Babesia infection; however, there was an occupation?specific difference detected in the sero?prevalence of Babesia infection (χ2] = 11.93，P < 0.05). Conclusion　Babesia infection is detected in voluntary blood donors in Jiangsu region, which should be paid much attention.