基于网络药理学探讨参芪五味子片治疗失眠的作用机制

目的：运用网络药理学方法探讨参芪五味子片治疗失眠的生物活性成分和潜在的作用机制。方法：从中药系统药理学数据库与分析平台（TCMSP）中收集参芪五味子片组方中药物的活性成分及靶点；使用Uniprot数据库预测并标准化活性成分的蛋白靶点，绘制出参芪五味子片中药-活性成分-靶点网络图，分析其核心成分；从Drugbank、OMIM以及DisGeNET疾病数据库中挖掘出失眠相关疾病靶点；将获得的药物靶点和疾病靶点取交集后，筛选出参芪五味子片治疗失眠的潜在作用靶点，然后利用收集到的靶点数据分别构建药物和疾病的蛋白质-蛋白质相互作用（PPI）网络并取其交集作为药物-疾病相互作用网络，运用网络拓扑学方法筛选出其核心靶点；最后，使用Metascape数据库对核心靶点进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析。结果：共获得63个药物活性成分、232个药物相关靶点及160个疾病靶点，其中有13个靶点是药物与疾病的共有靶点。将药物活性成分和疾病的交集PPI网络经过网络拓扑学分析得到55个核心靶点，将核心靶点进行富集分析后共得到779种GO功能条目和30条KEGG信号通路。结论：参芪五味子片可能通过抗炎、抗氧化、调节神经递质代谢、调节细胞周期等作用治疗失眠。

Mechanism of Shenqi Schisandra Tablets in the Treatment of Insomnia Based on Network Pharmacology

To study the bioactive components and potential mechanism of Shenqi Schisandra Tablets in the treatment of insomnia based on network pharmacology.Methods:The active components and targets of Shenqi Schisandra Tablets were collected from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).UniProt was used to predict and normalize the protein targets of active components.The herb-active component-target network of Shenqi Schisandra Tablets was established to predict the key components.The targets of insomnia were searched against Drugbank,Online Mendelian Inheritance in Man(OMIM),and DisGeNET.The common targets shared by the drug and disease were identified as the potential targets of Shenqi Schisandra Tablets for the treatment of insomnia.Further,the protein-protein interaction(PPI) network was established for the identification of core targets.Finally,Metascape was employed for the gene ontology(GO) annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment of the core targets.Results:The screening yielded 63 active components,232 targets corresponding to active components,and 160 disease-associated targets,of which 13 common targets were shared by Shenqi Schisandra Tablets and insomnia.The PPI network identified 55 core targets,for which 779 GO terms and 30 KEGG signaling pathways were enriched.Conclusion:Shenqi Schisandra Tablets may treat insomnia via anti-inflammatory effect,antioxidant activity,neurotransmitter metabolism regulation,and cell cycle regulation.