人胎肝细胞生长刺激物质与酪氨酸蛋白激酶的关系

利用从人胎肝提取的肝细胞生长刺激物质（ｈＨｓｓ），以小鼠肝细胞质膜蛋白作为受体及内源性底物，以Ｐｏｌｙ（ＧｌｕＮａ，Ｔｙｒ）４：１作为外源性底物，通过磷酸化实验，对ｈＨＳＳ的作用及其与酪氨酸蛋白激酶活性之间的关系进行了研究。利用抗酪氨酸磷酸酯抗体免疫沉淀法，ＳＤＳ－聚丙烯酰胺凝胶电泳和放射自显形及液体闪烁计数等方法进行测定。结果证明：ｈＨＳＳ可刺激并增强肝细胞膜受体的酪氨酸蛋白激酶的活性，后者可使膜蛋白某些组分及外源性底物磷酸化增强；ｈＨＳＳ的作用与酪氨酸蛋白激酶的活性密切相关。

The Studies on the Relationship Between Human Fetal Hepatic Stimulator Substance and the Activity or Tyrosin Kinase

HSS was isolated from human fetal liver. When stimulated by hHSS, plasma membrane of hepa-tocyte showed increased tyrosine phosphorylation of both endOgenous and extragenous substrates. Westudied the relationship between hHSS function and the activity of tyrosine kinase,using plasma mem-brane protein as endogenous substrate and Poly (GluNa, Tyr)4:1 as extragenous. The authors usedimmuno-precipitation with anti-phosphotyrosine antibody, SDS-PAGE,autoradiophotography,andliquid scintillation counting. The results revealed that hHSS specially increased the activity of tyrosinekinase in the plasma membrane:strengthened phosphorylation of its substrates at the tyrosine residue,and the function of hHSS was intimately related to the activity of tyrosine kinase.