DNA甲基转移酶1与CD11a基因在系统性红斑狼疮患者中mRNA水平的表达

 目的 检测系统性红斑狼疮（systemic lupus erythematosus，SLE）患者中DNA甲基转移酶1（DNA methyltransferase 1，DNMT1）和CD11a基因的表达水平，探讨DNMT1的表达异常在SLE发病中的作用。方法 用Real-time PCR方法测定SLE患者缓解期、活动期和正常人外周血单个核细胞（peripheral blood mononuclear cell，PBMC）中DNMT1和CD11a基因的表达水平。结果 SLE活动期患者PBMC中DNMT1的表达水平较对照组（P=0.014）和缓解期组（P=0.030）显著下降；缓解期的DNMT1表达水平与对照组（P=0.264）相比差异无统计学意义；SLE患者DNMT1的表达水平与SLE疾病活动指数（SLE disease activity index，SLEDAI）呈现负相关（P<0.05）。SLE活动期患者PBMC中CD11a的表达水平较对照组（P=0.003）显著升高（P<0.05）；缓解期的CD11a表达水平与对照组（P=0.250）和缓解期组（P=0.069）相比差异无统计学意义（P>0.05）；SLE患者CD11a的表达水平与SLEDAI呈正相关（P<0.05）。SLE患者DNMT1与CD11a的表达水平无显著相关性（P>0.05）。结论 SLE患者PBMC中DNMT1表达水平的降低可能是造成基因组DNA低甲基化的重要原因，使得CD11a等致病基因过度表达，进而导致SLE发病。

The mRNA expression of DNA methyltransferase 1 and CD11a in patients with systemic lupus erythematosus

Objective To detect the expression levels of DNMT1 and CD11a gene in systemic lupus erythematosus(SLE) patients,and investigate the role of abnormal expression of DNA methyltransferase 1(DNMT1) in the pathogenesis of SLE. Methods Real-time PCR was applied to detect the expression levels of DNMT1 and CD11a in peripheral blood mononuclear cells(PBMC) of patients with SLE in remission stage,active stage and healthy controls. Results The expression levels of DNMT1 in PBMC were significantly lower in active SLE ...