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江苏地区不同类型乙型肝炎患者HBV基因分型研究
引用本文:李桂珍,刘兴祥,王兴亮.江苏地区不同类型乙型肝炎患者HBV基因分型研究[J].第四军医大学学报,2007,28(2):157-159.
作者姓名:李桂珍  刘兴祥  王兴亮
作者单位:淮安市第四人民医院分子生物学实验室,江苏,淮安,223300
摘    要:目的:了解江苏地区乙型肝炎病毒基因型分布特征,探讨HBV各基因型与血清HBV DNA水平的关系.方法:采用荧光定量PCR结合Taqman MGB探针技术,对江苏地区176份乙型肝炎患者血清中的HBV DNA进行基因分型和定量检测.结果:176例血清中,C型117人(66.5%),B, C型40人(22.7%),B型9人(5.1%),D型1人(0.6%),A型1人(0.6%).C型与B, C混合型在HBV DNA水平上存在统计学差异(P<0.05),B, C混合型的HBV DNA水平明显高于C型.C基因型在慢性乙型肝炎患者中的所占比例(71.4%)比在急性乙型肝炎、肝硬化和肝癌患者中所占的比例较高,但差异无统计学意义.结论:江苏地区HBV基因型以C型为主, B, C混合型次之,B型较少,D型,A型极少;B, C混合型患者的HBV DNA数量水平高于C型.

关 键 词:肝炎病毒  乙型  基因型  聚合酶链反应
文章编号:1000-2790(2007)02-0157-03
修稿时间:2006-06-06

HBV genotyping in hepatitis B patients in Jiangsu province
LI Gui-Zhen,LIU Xing-Xiang,WANG Xing-Liang.HBV genotyping in hepatitis B patients in Jiangsu province[J].Journal of the Fourth Military Medical University,2007,28(2):157-159.
Authors:LI Gui-Zhen  LIU Xing-Xiang  WANG Xing-Liang
Abstract:AIM: To investigate the distribution of hepatitis B virus (HBV) genotypes of hepatitis B patients in Jiangsu province and analyze the relationship of HBV genotypes with the serum HBV DNA levels of patients. METHODS: The HBV genotypes and HBV DNA levels of 176 patients in Jiangsu province were detected by real-time quantitative PCR and Taqman MGB probe technology. RESULTS: Among 176 HBV-infected patients, the proportions of genotype C, B-C mixed-type, B, A and D were 66.5%(117/176), 22.7%(40/176), 5.1%(9/176), 0.6%(1/176) and 0.6%(1/176), respectively. The results showed there were significant differences between genotype C and genotype B-C mixed-type in HBV DNA load(P<0.05), and the HBV DNA level of genotype B-C mixed-type was significantly higher than that of genotype C. The rate of genotype C among the patients of chronic hepatitis B was 71.4%, higher than that among the patients of acute hepatitis B, liver cirrhosis and hepatocellular carcinoma, but it showed no significant differences in the various clinical types of HBV infection tested(P>0.05). CONCLUSION: Genotype C and genotype B-C mixed-type are the two dominant genotypes in the patients in Jiangsu province, and genotype B, A, and D are also found, but not common. The HBV DNA load of genotype B, C mixed-type is significantly higher than that of genotype C.
Keywords:Hepatitis B Virus  Genotype  polymerase chain reaction
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