| 2型猪链球菌荚膜唾液酸合成相关基因neuC敲除突变株的构建及其生物学特性 |
| |
| 引用本文： | 王长军,董瑞萍,潘秀珍,郑峰,李明,王晶,操敏,李先富,唐家琪.2型猪链球菌荚膜唾液酸合成相关基因neuC敲除突变株的构建及其生物学特性[J].中国病原生物学杂志,2010(4). |
| |
| 作者姓名： | 王长军 董瑞萍 潘秀珍 郑峰 李明 王晶 操敏 李先富 唐家琪 |
| |
| 作者单位： | 南京军区军事医学研究所; |
| |
| 基金项目： | 国家自然科学基金资助项目(No.30600533,No.30730081,No.30972638); 江苏省自然科学基金资助项目(No.BK2008066,No.BK2009042) |
| |
| 摘 要： | 目的探索荚膜多糖合成基因neuC与细菌唾液酸合成转化的关系。方法应用同源重组基因敲除方法构建筛选2型猪链球菌(SS2)强毒株05ZYH33荚膜多糖组分唾液酸合成相关基因neuC的缺失突变株;系统比较分析突变体与野生株的基本生物学特性的差异,小鼠和仔猪毒力试验分析neuC基因缺失对细菌毒力的影响。结果应用PCR检测和Southern杂交分析显示,neuC基因在2株转化重组体中被壮观霉素抗性基因所替代,表明neuC基因敲除突变体构建成功;突变体△neuC与野生株在菌落形态、染色特性和溶血活性方面无明显差异;突变体与2型特异性抗血清的凝集反应显著减弱;电镜观察显示突变体表面荚膜结构较野生株荚膜显著变薄,直径20～55nm,但质地更致密;小鼠和仔猪毒力试验显示,突变体毒力显著减弱。结论neuC是SS2荚膜多糖合成的重要结构基因,与细菌唾液酸的转化利用密切相关;neuC基因可能藉其在唾液酸转化中的作用影响SS2的基本生物学特性和细菌的侵袭致病能力。
|
| 关 键 词： | 猪链球菌2型(SS2) 尿苷二磷酸-N-乙酰葡糖胺-2-异构酶 基因敲除 致病性 |
Construction and functional analysis of the neuC gene knock-out mutant of Streptococcus suis serotype 2 |
| |
| WANG Chang-jun,DONG Rui-ping,PAN Xiu-zhen,ZHEN Feng,LI Ming,WANG Jing,CAO Min,LI Xian-fu,TANG Jia-qi.Construction and functional analysis of the neuC gene knock-out mutant of Streptococcus suis serotype 2[J].Journal of Pathogen Biology,2010(4). |
| |
| Authors: | WANG Chang-jun DONG Rui-ping PAN Xiu-zhen ZHEN Feng LI Ming WANG Jing CAO Min LI Xian-fu TANG Jia-qi |
| |
| Institution: | WANG Chang-jun,DONG Rui-ping,PAN Xiu-zhen,ZHEN Feng,LI Ming,WANG Jing,CAO Min,LI Xian-fu,TANG Jia-qi(Research Institute for Medicine of Nanjing Comm,,Nanjing 210002,China) |
| |
| Abstract: | Objective To construct and functionally analyze a mutant of the neuC gene encoding UDP-N-acetylglucosamine 2-epimerase of Streptococcus suis serotype 2(SS2)virulent strain 05ZYH33.Methods The neuC gene was knocked out based on the principle of homologous recombination.PCR analysis and Southern hybridization were used to identify the knockout.Transmission electron microscopy was employed to observe the morphology of the established strain.The virulence of the deficient strain was tested in piglet and murine ... |
| |
| Keywords: | Streptococcus suis serotype 2 UDP-N-acetylglucosamine 2-epimerase gene knock-out pathogenicity |
| 本文献已被 CNKI 等数据库收录！ |
|
