再障患者细胞因子与对应血象三系水平测定的临床意义

目的：评价再障患者外周血象白细胞（WBC）、红细胞（RBC）、血小板（PLT）三系数量与其各自对应的细胞因子水平的关系，探讨细胞因子对再生障碍性贫血（AA）发病的影响及其临床意义。方法：用放射免疫分析检测外周血中粒细胞-巨噬细胞集落刺激因子（GM—CSF）、白细胞介素-2（IL-2）、血小板生成素（TPO）水平；用化学发光免疫法检测外周血促红细胞生成素（EPO）；用全自动五分类血球计数仪检测血象。统计：所有数据采用SPSS10．0统计软件处理。结果：AA组外周血中WBC数：量、RBC数量、PLT数量及白细胞因子GM—CSF（P〈0．05）水平明显低于正常对照组；而IL-2、EPO、TPO（P〈0．05）细胞因子水平均明显高于正常对照组；GM—CSF与外周血WBC有正相关性，EPO与外周血RBC有一定的负相关性；TPO与PLT亦呈负相关。结论：再障患者外周血象兰系数量与对应细胞因子水平有一定的相关性。

Clinical Significance of Measurement of Plasma Relevant Cytokines (GM-CSF, IL-2, TPO, EPO) Levels in Patients with Aplastic Anemia

Objective To investigate the role of relevant cytokines in the development and pathogenesis of aplastic anemia. Methods Plasma GM-CSF, IL-2, TPO (with RIA) and EPO (with CLIA) contents were measured in 100 patients (acute 43, chronic 57) with aplastic anemia and 50 controls. Complete blood count was also performed in all these subjects. Results The peripheral RBC, WBC, platelet counts and GM-CSF contents were significantly lower in the patients with aplastic anemia than those in the controls (P<0.05), while the IL-2, EPO and TPO contents were significantly higher in the patients (P<0.05). GM-CSF contents were positively correlated with the WBC numbers. EPO contents were negatively correlated with the RBC counts and TPO contents were correlated (negatively) with the platelet counts. Conclusion There was correlationship between each blood elements (WBC, RBC, platelet) and its corresponding cytokine (GS-CSF, EPO, TPO respectively). IL-2 contents were not correlated with WBC counts.