Cloning and identification of a DNA fragment coding for the sup1 gene of Saccharomyces cerevisiae |
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| Authors: | Peter Breining Andrei P Surguchov Wolfgang Piepersberg |
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| Institution: | (1) Lehrstuhl für Mikrobiologie der Universität München, Maria-Ward-Straße 1a, D-8000 München 19, Germany;(2) Institute of Experimental Cardiology, USSR Research Centre of Cardiology, Academy of Medical Sciences, Cherepkovskaja 15A, 121552 Moscow, USSR |
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| Abstract: | Summary A plasmid, pYsup1-1, containing a DNA fragment able to suppress the recessive mutant phenotype of the suppressor locus sup1 (allele sup1-ts36) of Saccharomyces cerevisiae was isolated from a bank of yeast chromosomal DNA cloned in cosmid p3030. The complementing gene was localized on a 2.6 kb DNA fragment by further subcloning. Evidence is presented that the cloned DNA segment codes for the sup1 structural gene (chromosome IIR). |
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| Keywords: | Saccharomyces cerevisiae Cloning Suppressor |
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