| 维甲酸对HeLa细胞间隙连接蛋白基因cx43表达的调节作用 |
| |
| 引用本文： | 陈必良,马向东,王德堂,辛晓燕.维甲酸对HeLa细胞间隙连接蛋白基因cx43表达的调节作用[J].癌症,1999,18(6):646-648,656. |
| |
| 作者姓名： | 陈必良 马向东 王德堂 辛晓燕 |
| |
| 作者单位： | 第四军医大学西京医院妇产科,西安,710032 |
| |
| 摘 要： | 目的：探讨分化诱导剂维甲对肿瘤抑制基因－细胞间隙连接蛋白基因cx４３在人子宫颈癌细胞系ＨeＬa中表达的调节作用。方法：应用核酸原位杂交、流式细胞仪、Ｗesten blot及Ｌucifer Ｙellow划痕标记荧光传输技术，研究维甲酸作用对ＨeＬa细胞cx４３mＲＮＡ及其蛋白表达，以及对ＨeＬa细胞生长和通迅功能找调节作用。结果：ＨeＬa细胞经维甲酸处理后，原位杂交显示，ＨeＬa细胞cx４３ mＲＮＡ水平上调；流式细胞仪分析，Ｃx４３蛋白
|
| 关 键 词： | 间隙连接 HeLa细胞 维甲酸 原位杂交 流式细胞仪 细胞间隙连接通讯 |
| 文章编号： | 1000－467(1999)06－0646－03 |
The regulation effect of all-trans-retinoic acid on expression of connexin gene cx43 in HeLa |
| |
| CHEN Bi liang,MA Xiang dong,WANG De tang,et al..The regulation effect of all-trans-retinoic acid on expression of connexin gene cx43 in HeLa[J].Chinese Journal of Cancer,1999,18(6):646-648,656. |
| |
| Authors: | CHEN Bi liang MA Xiang dong WANG De tang |
| |
| Institution: | CHEN Bi liang,MA Xiang dong,WANG De tang,et al. Department of Obstetric & Gynecology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,P.R. China$$$$ |
| |
| Abstract: | Objectives:To investigate the expression of connexin gene (cx43)in human cervical carcinoma cell line HeLa, and the regulation effect of all trans retinoic acid (ATRA).Methods:In situ hybridization (ISH),flowcytometer (FCM),Western blot and Lucifer Yellow scrape loading and dye transfer (SLDT) were used to detect the expression of cx43 mRNA and protein in HeLa cell,and the regulation effect of ATRA. Results:After treatment with ATRA,HeLa cell grew much slowly than untreated cell.ISH showed cx43 mRNA detectable and up regulation.FCM and Western blot found the positive rate of cx43 in 43KDa protein in HeLa cells increasing from 1.9%to 26.3%.Gap junction intracellular communication (GJIC) recovered in RA treated cell was more than untreated cell. Conclusion:The anti tumor effect of ATRA on HeLa Cell might be due to up regulation of cx43 gene and recovery of cx mediated GJIC. |
| |
| Keywords: | Connexin Cervical neoplasm Retinoic acid In situ hybridization Flowcytomeer Gap junction intracellular communication |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
