幽门螺杆菌相关胃溃疡的细胞增殖与凋亡研究

目的观察Ｈｐ感染对胃溃疡（ＧＵ）患者胃粘膜上皮细胞增殖与凋亡的影响，进而对溃疡发生机制进行探讨．方法标本取自内镜下胃粘膜活检，细胞凋亡检测采用Ｔｕｎｅｌ方法，细胞增殖检测采用免疫组化ＬＳＡＢ方法测定增殖细胞核抗原（ＰＣＮＡ）．结果ＧＵＨｐ＋患者细胞凋亡指数（ＡＩ）明显高于Ｈｐ－患者（Ｐ＜００５），但比较两者的增殖细胞指数（ＰＣＮＡＬＩ），无显著性差异（Ｐ＞００５）；慢性浅表性胃炎Ｈｐ＋患者ＡＩ，ＰＣＮＡＬＩ明显高于Ｈｐ－患者（Ｐ＜００５），ＧＵＨｐ＋患者的ＡＩ／ＰＣＮＡＬＩ比值为１５４１，明显高于其他各组（Ｐ＜００５）．结论Ｈｐ感染能诱导ＧＵ患者胃粘膜上皮细胞凋亡增加，出现细胞凋亡与细胞增殖平衡失调，促进溃疡形成

Studies on gastric epithelial cell proliferation and apoptosis in Hp associated gastric ulcer

AIM To investigate whether Helicobacter pylori (Hp) infection has any effects on the gastric epithelial cell proliferation and apoptosis,as well as its role in the pathogenesis of gastric ulcer. METHODS Endoscopic gastric mucosal biopsies were taken from 1 group (10 cases) of normal subjects (NS) and 4 groups (10 cases for each group) of patients with gastric ulcer (GU) and chronic superficial gastritis (CSG) with or without Hp infection. Apoptosis cells in gastric mucosa were quantitated by terminal deoxynucleatidyl transferase mediated dUTP nick and labelling (Tunel method) and proliferation cells by immunohistochemical staining (LSAB method) for proliferating cell nuclear antigen (PCNA). RESULTS Apoptosis index (AI) of the patients in Hp positive GU group was much higher than that of the patients in Hp negative GU group ( P <0 05), but there was no significant difference in PCNA labelling indices (PCNA LI) between the two groups ( P >0 05). AI and PCNA LI of the patients in Hp positive CSG group were significantly higher than those of the patients in Hp negative CSG group ( P <0 05). AI/PCNA LI ratio of the patients in Hp positive GU group was 1 541, and this ratio was much higher than those in other groups ( P <0 05). CONCLUSION Hp infection might induce apoptosis of gastric epithelial cells, and this effects might lead to the imbalance of the AI/PCNA LI ratio and ultimately promote the development of gastric ulcer.