An explanation of the noncorrespondence between assessment methods of cyclosporin |
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| Authors: | G M Randleff-Rasmussen M M J Oosthuizen J A Myburgh |
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| Institution: | (1) Biochemistry Laboratory, Department of Surgery, University of the Witwatersrand Medical School, 7 York Road, Parktown, 2193 Johannesburg, South Africa |
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| Abstract: | Various methods of determining cyclosporin (CyA) levels in patients after kidney transplantation were compared. These included
polyclonal antibody (pcAb-), specific and nonspecific monoclonal antibody (S- and NmcAb-) radioimmunoassays (RIA), and high
performance liquid chromatography (HPLC). The results obtained by the various methods when compared showed some correlation
but did not correspond. A probable explanation for part of this noncorrespondence is the presence of monoclonally crossreactive
metabolites (CyA-M). Another reason was that the concentration of CyA in the standards supplied with the RIA kits was found
to be higher than stated. |
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| Keywords: | Cyclosporin determination in kidney transplantation Cyclosporin determination radioimmunoassay HPLC Radioimmunoassay of cyclosporin HPLC of cyclosporin |
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