大黄素、生长抑素对急性胰腺炎大鼠肠黏膜细胞的保护机制

目的: 探讨急性胰腺炎大鼠肠黏膜细胞膜的流动性和细胞膜"钙泵"活性的变化机制,并研究大黄素和生长抑素对上述变化影响。方法: 雄性SD大鼠80只,随机分为假手术组、生理盐水处理组、大黄素处理组、生长抑素处理组,5%牛磺胆酸钠逆行性胆胰管注射制作急性胰腺炎大鼠模型,制模6 h后测定大鼠回肠组织细胞膜钙泵活性、荧光偏振度值、丙二醛含量及回肠组织黏膜血流量。结果: 与生理盐水处理组相比较,大黄素处理组和生长抑素处理组的丙二醛含量降低(P<0.01),细胞膜钙泵活性、细胞膜流动性增加(P<0.01),肠黏膜缺血得到显著改善。结论: 大黄素和生长抑素可通过改善肠黏膜缺血、提高钙泵活性并改善肠黏膜细胞膜流动性,从而起到保护肠黏膜屏障的作用。

Role of emodin and somatostatin in protection of intestinal mucosal barrier cells in rats with acute pancreatitis

Objective:To explore the alterative mechanism of intestinal mucosal cell membrane fluidity and calcium pump in rats with acute pancreatitis(AP),and to study the effects of emodin and somatostatin on them.Methods:Eighty male SD rats were randomly divided into four groups:sham operation group(SO),normal saline(NS) group,emodin group and somatostatin group.Intestinal mucosal cell membrane Ca~(2+)-Mg~(2+)ATPase activity,fluorescence polarization,malondialdehyde(MDA) content and blood flow of intestinal mucosa were determined 6 hours after the model rats of acute pancreatitis were established.Results:In the emodin group and somatostatin group,the MDA content was lower than that in the SO group(P<0.01),Ca~(2+)-Mg~(2+)ATPase activity and membrane fluidity were higher than those in SO group(P<0.01)and the decreased value of blood flow of intestinal mucosa was lower compared with that of SO group.Conclusions:Emodin and somatostatin can protect the function of intestinal mucosal barrier by ameliorating intestinal mucosa ischemia,improving calcium pump activity and elevating intestinal mucosal cell membrane fluidity.