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| 硒对人脐静脉内皮细胞ECV-304单核细胞趋化蛋白-1表达的影响 | |
| 引用本文： | 李艳波,邓华聪,郑丹,李呼伦,周宏博. 硒对人脐静脉内皮细胞ECV-304单核细胞趋化蛋白-1表达的影响[J]. 中国地方病学杂志, 2003, 22(3): 221-222 |
| 作者姓名： | 李艳波 邓华聪 郑丹 李呼伦 周宏博 |
| 作者单位： | 1. 重庆医科大学,附属一院,内分泌科,重庆400016 2. 哈尔滨医科大学,微生物学教研室,黑龙江,哈尔滨,150086 3. 哈尔滨医科大学,生物化学教研室,黑龙江,哈尔滨150086 |
| 基金项目： | 国家自然科学基金资助项目 (3 0 0 70 3 5 6) |
| 摘 要： | 目的 观察硒对人脐静脉内皮细胞ECV-304单核细胞趋化蛋白-1(MCP-1)表达的影响方法分别用高葡萄糖、糖基化终末产物，高胰岛素和过氧化氢孵育人脐静脉内皮细胞ECV-304；在先加入100nmol／L硒后，再给予以上4种因素．分别检测人脐静脉内皮细胞ECV0304MCP-1mRNA的表达并比较。结果 4种因素均可作为独立因素，导致人脐静脉内皮细胞ECV304MCP-1mRNA表达量增加：硒能抑制4种因素所致的MCP-1mRNA表达。结论 硒抑制MCP-1 mRNA在人脐静脉内皮细胞ECV-304中的表达。 |
| 关 键 词： | 硒 脐静脉 内皮细胞 ECV-304 单核细胞趋化蛋白-1 动脉粥样硬化 抗氧化剂 |
| 文章编号： | 1000-4955(2003)03-0221-02 |
| 修稿时间： | 2002-10-07 |
Effect of selenium on monocyte chemoattractant protein-1 in human umbilical vein endothelial cells |
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| LI Yan-bo ,DENG Hua-cong ,ZHENG Dan ,LI Hu-lun ,ZHOU Hong-bo. Effect of selenium on monocyte chemoattractant protein-1 in human umbilical vein endothelial cells[J]. Chinese Jouranl of Endemiology, 2003, 22(3): 221-222 | |
| Authors: | LI Yan-bo DENG Hua-cong ZHENG Dan LI Hu-lun ZHOU Hong-bo |
| Affiliation: | LI Yan-bo 1,DENG Hua-cong 1,ZHENG Dan 1,LI Hu-lun 2,ZHOU Hong-bo 3 |
| Abstract: | Objective To observe the effect of selenium on expression of monocyte chemoattractant protein-1 (MCP-1) mRNA in human umbilical vein endothelial cell line ECV-304.Methods Cell line ECV-304 was incubated with high glucose, advanced glycosylation end products (AGE), high insulin and H 2O 2 respectively;simultaneously, the cell line ECV-304 pre-treated with selenium was incubated with above factors, the expression of MCP-1 mRNA was detected and compared.Results Four factors increased the expression of MCP-1 mRNA independently;selenium inhibited the expression of MCP-1 mRNA induced by 4 factors distinctly.Conclusions Selenium repress the expression of MCP-1 mRNA in the cell line ECV-304. |
| Keywords: | selenium monocyte chemoattractant protein-1 human umbilical vein endothelial cells |
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