| 人生长终止特异性同源盒基因重组腺病毒载体的构建及鉴定 |
| |
| 引用本文： | 郑辉,薛松,黄日太,胡振雷,汪永义. 人生长终止特异性同源盒基因重组腺病毒载体的构建及鉴定[J]. 医学研究生学报, 2011, 24(3) |
| |
| 作者姓名： | 郑辉 薛松 黄日太 胡振雷 汪永义 |
| |
| 作者单位： | 上海交通大学附属仁济医院心胸外科,上海,200127 |
| |
| 摘 要： | 目的 生长终止特异性同源盒(growth arrest-specific homeobox,Gax)基因表达产物是核转录因子,主要存在于心血管系统中,对胚胎发育、细胞生长、分化和迁移等起基因调控作用,其表达异常与多种疾病相关.为深入研究Gax基因在临床疾病中的作用,文中构建表达了人Gax基因的重组腺病毒载体.方法 根据pEGFP-N1-Gax质粒的多克隆位点,用NheⅠ和Hind Ⅲ双酶切获得人Gax基因片段,连接至穿梭质粒pDC18-mCMV上,构建穿梭质粒pDC318-mCMV-Gax,然后与骨架质粒pPE3共转染293包装细胞,同源重组产生复制缺陷型重组腺病毒载体Ad5-Gax,反复感染293细胞扩增病毒后,氯化铯梯度离心纯化病毒,并测定病毒滴度.结果 穿梭质粒pDC318-mCMV-Gax经双酶切和测序证实构建成功;PCR鉴定人Gax基因重组腺病毒载体构建成功,病毒滴度为4.5×1010pfu/ml.结论 该研究成功构建了重组腺病毒载体Ad5-Gax,为进一步在体内外研究Gax基因参与多种疾病的发生发展提供了材料.
|
| 关 键 词： | 生长终止特异性同源盒基因 腺病毒载体 基因重组 |
Construction and identification of human growth arrest-specific homeobox gene recombinant adenovirus |
| |
| ZHENG Hui,XUE Song,HUANG Ri-tai,HU Zhen-lei,WANG Yong-yi. Construction and identification of human growth arrest-specific homeobox gene recombinant adenovirus[J]. Bulletin of Medical Postgraduate, 2011, 24(3) |
| |
| Authors: | ZHENG Hui XUE Song HUANG Ri-tai HU Zhen-lei WANG Yong-yi |
| |
| Affiliation: | ZHENG Hui,XUE Song,HUANG Ri-tai,HU Zhen-lei,WANG Yong-yi (Department of Cardiothoracic Surgery,Renji Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200127,China) |
| |
| Abstract: | Objective The nuclear factor,a product of the growth arrest-specific homeobox(Gax) gene expression,mainly exists in the cardiovascular system and is involved in the gene regulation of embryonic development and cell growth,differentiation and migration.The abnormal expression of Gax might be associated with the pathogenesis of many diseases.This study aimed at a deeper insight into the role of Gax in the progression of clinical diseases by constructing a recombinant adenovirus vector carrying the human Gax g... |
| |
| Keywords: | Grow arrest-specific homeobox gene Adenovirus vector Gene recombination |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
