| 应用DNA芯片法检测耐多药结核分枝杆菌的研究 |
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| 引用本文： | 李锋,李凫坚,陈园园,王静. 应用DNA芯片法检测耐多药结核分枝杆菌的研究[J]. 医学研究杂志, 2011, 40(1): 115-117 |
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| 作者姓名： | 李锋 李凫坚 陈园园 王静 |
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| 作者单位： | 浙江省中西医结合医院结核科,杭州,310003 |
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| 摘 要： | 目的应用DNA芯片技术快速检测耐多药结核菌的常见突变位点,初步评价其临床应用价值。方法以结核分枝杆菌标准株H37RV为对照,应用基因芯片技术、聚合酶链反应-单链构象多态性技术、聚合酶链反应-直接测序技术检测46株耐多药结核分枝杆菌临床分离株katG、rpoB基因。结果 46例耐多药菌株中,应用基因芯片检测出katG基因突变28株,检出率为60.9%(28/46);检测出rpoB基因突变37株,检出率为80.4%(37/46);同时检测出二者突变共25株,检出率为54.3%(25/46)。结论基因芯片技术操作简单、检测快速,对异烟肼、利福平耐药基因检出率较高,可作为临床耐药检测的辅助手段。
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| 关 键 词： | 结核 耐药 基因 芯片 |
| 收稿时间： | 2010-07-12 |
Rapid Detection of Multidrug-resistant Mycobacterium Tuberculosis by DNA Chip |
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| Li Feng,Li Fujian,Chen Yuanyuan,Wang Jing. Rapid Detection of Multidrug-resistant Mycobacterium Tuberculosis by DNA Chip[J]. Journal of Medical Research, 2011, 40(1): 115-117 |
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| Authors: | Li Feng Li Fujian Chen Yuanyuan Wang Jing |
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| Affiliation: | Li Feng,Li Fujian,Chen Yuanyuan,Wang Jing.Department of Tuberculosis,Integrated Chinese and Western Medicine Hospital of Zhejiang Province,Zhejiang 310003,China |
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| Abstract: | Objective To develop and evaluate a new DNA chip diagnosis technique for rapid detection of multidrug-resistant mycobacterium tuberculosis isolated from clinical laboratorial specimen.Methods Compared with the standard strain,46 multidrug-resistant strains were detected by oligonucleotide microchips,PCR-SSCP and analyzed by direct sequencing technique.Results katG mutations were found in 28 strains(60.9%,28/46) detected by DNA chips,rpoB mutations positive in 37 strains(80.4%,37/46),katG and rpoB simultaneo... |
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| Keywords: | Tuberculosis Drug-resistant Gene Chips |
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