| A(H1N1)甲型流感病毒基因多态性对病毒复制及毒力的影响研究 |
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| 引用本文： | 玛依拉,许黎黎,鲍琳琳,吕琦,邓巍,李枫棣,占玲俊,朱华,马春梅,秦川. A(H1N1)甲型流感病毒基因多态性对病毒复制及毒力的影响研究[J]. 医学研究杂志, 2011, 40(4): 23-26 |
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| 作者姓名： | 玛依拉 许黎黎 鲍琳琳 吕琦 邓巍 李枫棣 占玲俊 朱华 马春梅 秦川 |
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| 作者单位： | 中国医学科学院实验动物研究所、北京协和医学院比较医学中心、卫生部人类疾病比较医学重点实验室、国家中医药管理局人类疾病动物模型三级实验室,北京,100021 |
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| 基金项目： | 国家"十一五"科技重大专项--艾滋病和病毒性肝炎等重大传染病防治，卫生部卫生公益性行业科研专用项目 |
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| 摘 要： | 目的研究A(H1N1)甲型流感病毒基因多态性对病毒复制及毒力的影响。方法选择A/Californ ia/04/2009,A/S ichuan/1/2009和A/Be ijing/3/2009 3株病毒,分别感染MDCK细胞,测定病毒RNA载量和病毒效价;同时滴鼻接种BALB/c小鼠,检测小鼠感染后的多项指标,观察期为14天。另一方面,对3株病毒的全基因组进行测序及序列比对,对其基因突变情况及多态性进行分析。结果 A/S ichuan/1/2009株相较而言具有较高的复制能力及毒力,序列分析结果显示全基因中有5个氨基酸发生了突变,其中PA蛋白中343位点的A突变为T,PB1蛋白中353位点的K突变为R,566位点的T突变为A,PB2蛋白中471位点的T突变为M,对病毒毒力的增强起重要作用。结论 A(H1N1)甲型流感病毒基因组多态性与其复制及毒力密切相关。
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| 关 键 词： | A(H1N1)甲型流感病毒 多态性 复制 毒力 |
| 收稿时间： | 2010-12-29 |
| 修稿时间： | 2011-02-18 |
Effect of Gene Polymorphism of A (H1N1) Influenza Virus on Viral Replication and Virulence |
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| Ma Yila,Xu Lili,Bao Linlin,Lü Qi,Deng Wei,Li Fengdi,Zhan Lingjun,Zhu Hua,Ma Chunmei,Qin Chuan. Effect of Gene Polymorphism of A (H1N1) Influenza Virus on Viral Replication and Virulence[J]. Journal of Medical Research, 2011, 40(4): 23-26 |
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| Authors: | Ma Yila Xu Lili Bao Linlin Lü Qi Deng Wei Li Fengdi Zhan Lingjun Zhu Hua Ma Chunmei Qin Chuan |
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| Affiliation: | Ma Yila,Xu Lili,Bao Linlin,Lü Qi,Deng Wei,Li Fengdi,Zhan Lingjun,Zhu Hua,Ma Chunmei,Qin Chuan.Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS) & Comparative Medicine Center,Peking Union Medical Collage(PUMC),Key Laboratory of Human Disease Comparative Medicine,Ministry of Health,Key Laboratory of Human Diseases Animal Model,State Administration of Traditional Chinese Medicine,Beijing 100021,China |
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| Abstract: | Objective To study the effect of gene polymorphism of A(H1N1) influenza virus on viral replication and virulence.Methods Three strains of 2009 pandemic A(H1N1) influenza viruses with diverse clinical phenotypes were investigated.Virus replication was analyzed in MDCK cells in vitro,whereas virulence and pathogenicity were examined using BALB/c mice model in vivo.The genetic basis for increasing virulence of the 2009 pandemic A(H1N1) viruses was assessed,and multiple amino acid residues were supposed to be i... |
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| Keywords: | A(H1N1) influenza virus Polymorphism Replication Virulence |
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