| CD146短发夹RNA真核表达载体的构建与鉴定 |
| |
| 引用本文： | 陈伟,董震,蒋勇,苏寒,刘宝林,孙沫逸.CD146短发夹RNA真核表达载体的构建与鉴定[J].医学研究生学报,2011,24(3). |
| |
| 作者姓名： | 陈伟 董震 蒋勇 苏寒 刘宝林 孙沫逸 |
| |
| 作者单位： | 1. 南京军区南京总医院口腔科,南京,210002
2. 第四军医大学口腔医学院,西安,710032 |
| |
| 基金项目： | 国家自然科学基金，南京军区南京总医院青年基金 |
| |
| 摘 要： | 目的 CD146基因表达增高与涎腺腺样囊性癌(adenoid cystic carcinoma,ACC)嗜神经侵袭(perineural invasion,PNI)的临床症状相关.为进一步研究CD146基因的功能,构建人CD146短发夹RNA(short hairpin RNA,shRNA)真核表达载体并鉴定.方法 根据Genebank中靶基因CD146序列,按照Tuschl设计原则,设计、合成编码其shRNA的互补寡核苷酸序列,克隆至线性化的pGenesil-1载体中并对重组质粒进行酶切分析及测序鉴定.结果 经过限制性内切酶酶切分析和DNA测序鉴定,证实重组质粒pGe-CD146-shRNA的序列正确.结论 成功构建了靶向人CD146基因的shRNA真核表达载体系统,为进一步研究CD146基因的功能奠定了实验基础.
|
| 关 键 词： | 短发夹RNA 真核表达载体 |
Construction and identification of the eukaryotic expression vector expressing short hairpin RNA targeting human CD146 |
| |
| CHEN Wei,DONG Zhen,JIANG Yong,SU Han,LIU Bao-lin,SUN Mo-yi.Construction and identification of the eukaryotic expression vector expressing short hairpin RNA targeting human CD146[J].Bulletin of Medical Postgraduate,2011,24(3). |
| |
| Authors: | CHEN Wei DONG Zhen JIANG Yong SU Han LIU Bao-lin SUN Mo-yi |
| |
| Institution: | CHEN Wei1,DONG Zhen1,JIANG Yong1,SU Han1,LIU Bao-lin2,SUN Mo-yi2 |
| |
| Abstract: | Objective The overexpression of CD146 is reportedly associated with perineural invasion in salivary adenoid cystic carcinoma.This study was designed to construct and identify the eukaryotic expression vector expressing short hairpin RNA(shRNA) targeting human CD146.MethodsTwo pairs of shRNA were designed according to the human CD146 sequence in the Genebank(NM_006500) following the rules of Tuschl,and then synthesized and cloned into the linearized pGenesil-1 vector.The recombinant vector was confirmed by e... |
| |
| Keywords: | CD146 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
