支气管哮喘BALB/c小鼠血小板P-选择素表达的变化

目的观察支气管哮喘小鼠的血小板表面抗原P-选择素表达的变化。方法随机将雌性BALB/c小鼠20只分为：正常对照组、哮喘模型组；用流式细胞仪测定血小板膜上P-选择素的阳性率。结果正常对照组和哮喘组P-选择素表达的阳性率分别为（1．23±1.02）%、（4.52±1.45）%，哮喘模型组P-选择素较正常对照组增高，差异有统计学意义（P<0.01）；凝血酶诱导P-选择素数值正常对照组和哮喘模型组分别为（45.12±4.03）%、（44.68±5.34）%，差异无统计学意义（P>0.05）；正常对照组和哮喘组肺泡灌洗液中性粒细胞分别为（6.64±1.86）%、（25.60±2.66）%，差异有统计学意义（P<0.01）。结论支气管哮喘小鼠中存在血小板的活化，活化后的血小板可能参与哮喘的炎症反应。

Expression of P-selectin deprivation from the platelets of asthmatic BALB/c mice

To explore the expression of P-selectin derived from platelets of asthmatic mice. Methods Female BABL/c mice were randomly divided into two groups: the control group and the asthma group, and the expression of P-selectin was determined by flow cytometer (FCM). ResultsPositive expression of P-selectin rates in the control group and the asthma group were（1．23±1.02）% and （4.52±1.45）%（P<0.01）, but after thrombin activation, they were（45.12±4.03）% and（44.68±5.34）% (P>0.05). The leukocyte counts of bronchial alveo lavage(BAL) in the control group and the asthma group were 6.64±1.86 and 25.60±2.66（P<0.01）. Conclusion Platelet -activation exists in asthmatic mice and may participate in the asthmatic process.