Abbott RealTime PCR assay is useful for evaluating virological response to antiviral treatment for chronic hepatitis C |
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Authors: | Hiroaki Ikezaki Norihiro Furusyo Takeshi Ihara Takeo Hayashi Eiichi Ogawa Kazuhiro Toyoda Hiroaki Taniai Mosaburo Kainuma Masayuki Murata Jun Hayashi |
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Institution: | (1) Department of General Internal Medicine, Kyushu University Hospital, Higashi-Ku, Fukuoka 812-8582, Japan;(2) Department of Environmental Medicine and Infectious Disease, Kyushu University, Fukuoka, Japan |
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Abstract: | This study was done to evaluate the utility of the Abbott RealTime PCR assay (ART) for the monitoring of chronic hepatitis
C patients. The serum samples of 183 patients infected with hepatitis C virus (HCV) genotype 1b who had completed a 48-week
period of pegylated interferon (PEG-IFN) alpha-2b plus ribavirin treatment were prospectively analyzed. Serum HCV RNA levels
were measured both by ART and by the Roche COBAS Amplicor Monitor test, version2.0 (CAM) at baseline and at weeks 4, 12, 24,
36, and 48 of treatment, and at 24 weeks after the end of treatment (EOT). A significant positive correlation of pretreatment
HCV RNA levels was found between ART and CAM (r = 0.595, P < 0.0001). Of the 183 patients, 66 (36.0%) achieved a sustained virological response (SVR). The logarithmic decline of the
HCV RNA level from the pretreatment level determined by ART in SVR patients was significantly higher than that in non-SVR
patients at all time points tested. The logarithmic decline determined by CAM in SVR patients was significantly higher than
that in non-SVR patients only at week 4, but there was no significant difference at other weeks. Of 124 patients who were
HCV RNA-negative at EOT by ART, 58 (46.8%) had a relapse of viremia at 24 weeks after EOT, whereas 77 of 143 patients (53.8%)
who were HCV RNA-negative at EOT by CAM had a relapse. The relapse rate was lower when determined by ART than by CAM, but
not significantly so. ART is more useful than CAM for evaluating the virological response to antiviral treatment for chronic
hepatitis C. |
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Keywords: | Hepatitis C Interferon Ribavirin RealTime PCR |
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