| Ghrelin对高糖诱导心肌细胞凋亡的影响 |
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| 引用本文： | 张颖,肖谦. Ghrelin对高糖诱导心肌细胞凋亡的影响[J]. 中国老年学杂志, 2010, 30(6) |
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| 作者姓名： | 张颖 肖谦 |
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| 作者单位： | 重庆医科大学附属第一医院老年科内分泌组,重庆,400016 |
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| 摘 要： | 目的 探讨Ghrelin对高糖诱导心肌细胞凋亡的影响.方法 将原代培养的心肌细胞随机分为:①正常对照组(N组);②高糖组(H组);③高糖+Ghrelin组(G组).应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测各组心肌细胞凋亡,分光光度法检测心肌细胞内caspase-3活性的变化.结果 与N组比较,H组心肌细胞凋亡明显增加,caspase-3活性显著升高;与H组比较,G组心肌细胞凋亡数量明显减少,caspase-3活性明显降低(均P＜0.05).结论 Ghrelin可能通过降低caspase-3活性抑制高糖诱导的心肌细胞凋亡.
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| 关 键 词： | 高血糖 心肌细胞 凋亡caspase-3 |
The effects of Ghrelin on hyperglycemia-induced apoptosis in cultured cardiomyocyte |
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| ZHANG Ying,XIAO Qian. The effects of Ghrelin on hyperglycemia-induced apoptosis in cultured cardiomyocyte[J]. Chinese Journal of Gerontology, 2010, 30(6) |
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| Authors: | ZHANG Ying XIAO Qian |
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| Affiliation: | ZHANG Ying,XIAO QianEndocrinology Group,Department of Geriatrics,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China |
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| Abstract: | Objective To investigate the effects of Ghrelin on hyperglycemia-induced apoptosis in cultured rat cardiomyocyte.Methods The cultured cardiomyocytes were randomly divided into Group N (cardiomyocytes exposed to low glucose level,5.5 mmol/L);Group H (cardiomyocytes were cultured with high glocose level,25.0 mmol/L);Group G(cardiomyocytes were cultured in both high glucose level,25.0 mmol/L and Ghrelin).Apoptosis in different groups was determined by TUNEL.The change of caspase-3 activity in cardiomyocytes wa... |
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| Keywords: | Ghrelin Ghrelin High glucose Cardiomyocyte Apoptosis Caspase-3 |
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