肝硬变门静脉高压症患者肝细胞凋亡与原癌基因表达的研究

目的探讨肝细胞凋亡与肝硬变及原癌基因的关系。方法取正常人及肝硬变门静脉高压症患者的肝组织。行透射电镜检查，并检测其凋亡细胞及原癌基因表达。结果肝硬变患者肝组织内的凋亡细胞及凋亡指数高于正常人；肝组织原癌基因c-myc，c-fos和bcl-2均呈过度表达，而正常人肝组织则无表达。结论肝细胞凋亡在肝硬变的演变过程中发挥一定作用，原癌基因c-myc，c-fos和bcl-2参与肝细胞凋亡的调控。

A study of hepatocyte apoptosis and its gene expression in patients with liver cirrhosis and portal hypertension

Objective To study the relationship of hepatocyte apoptosis and gene expression of liver cirrhosis and primary hepatic carcinoma. Methods Specimens of liver tissue from patients with liver cirrhosis and portal hypertension and normal subjects were examined by transmission electron microscope to detect apoptotic cells and primary cancer gene expression. Results The number of apoptotic cells and the apoptotic index of cirrhotic liver tissue was higher than that of normal subjects.Immunostaining of bcl 2,c myc,c fos was negative in normal liver tissue ,but was over expressed in cirrhosis. Conclusions Hepatocyte apoptosis plays a certain role in the development of hepatic cirrhosis. The oncogenes bcl 2,c myc,c fos take part in the regulation of hepatocyte apoptosis .