联合移植修复长段骨缺损的实验研究

[目的 ]探讨肌肉瓣与骨髓加松质骨颗粒联合移植修复长段骨缺损中碱性成纤维细胞生长因子 (bFGF)及转化生长因子 (TGF β)的表达及修复机理 .[方法 ]在健康中国家兔双侧桡骨中段制作 2 0mm的长骨缺损模型 ,实验组行肌肉瓣与骨髓加松质骨颗粒联合移植 ,对照组单纯植入肌肉瓣 .不同时期进行各组家兔的组织学观察以及bFGF与TGF β多克隆抗体的免疫组织化学染色 .[结果 ]行移植术后 2 ,4 ,6 ,8周时实验组家兔软骨细胞、成骨细胞及骨细胞的bFGF及TGF β呈强阳性染色 .对照组家兔在移植修复 4周时肌肉组织间形成的软骨细胞中有少量软骨细胞呈阳性染色 ,6周及 8周骨缺损两端新生骨痂中部分骨细胞的bFGF及TGF β呈阳性染色 ,但阳性染色细胞数与实验组相比有显著性差异 .[结论 ]肌肉瓣与骨髓加松质骨颗粒联合移植早期在骨缺损局部中bFGF与TGF β的表达与分泌旺盛 ,促进诱导成骨 ,较快地修复长段骨的缺损 .

Exprement study on repairing the long bone defects with combined transplantation

OBJECTIVETo study the expression and mechanism of basic fibroblast grouth factor(bFGF) and transforming grouth factor(TGF-β) on repairing the long bone defect with transplanting complex muscle flap, bone marrow and granule of cancellous bone.METHODSThe 20mm of bone defect models were made in each side of radius, and muscle flap, bone marrow and granule of cancellous bone were transplanted in experimental group, and muscular flaps were transplanted in control group.The hitological staining and immunohistochemistry staning of bFGF and TGF-β were observed in the different session.RESULTSThe chondrocyte, osteoblast and osteocyte were highly expressed bFGF and TGF-β in experimental group after third days second, 4th, 6th, 8th weeks of operation. In control group, bFGF and TGF-β were expressed in small number of chondrocyte after 4 weeks of operation, and that were expressed in osteocyte of the new formed bone of cut ends after 6th and 8th weeks of operation, but the number of expressing cells are more than that of experimental group significantly.CONCLUSIONThe bFGF and TGF-β in the part of bone defect were highly expressed and released in combined transplantation of muscular flap, bone marrow and granule of cancellous bone in the early stage, and the bone defect can be repaired earliy and better by that.