Cystatin M在乳腺癌中的表达及其临床意义

目的 了解cystatin M在乳腺肿瘤中的表达及其临床意义.方法 采用实时定量PCR检测52例乳腺肿瘤标本及邻近正常乳腺组织中cystatin M mRNA及内参GAPDH表达水平,分析cystatin M基因表达与临床病理参数的关系.结果 乳腺癌标本的cystatin M含量与其周围邻近的正常乳腺组织及良性肿瘤中表达cystatin M含量无统计学意义;但发生淋巴结转移的乳腺肿瘤标本的cystatin M则明显低于其周围邻近的正常乳腺组织(P＜0.05),也明显低于未发生淋巴结的乳腺肿瘤标本(P＜0.05);乳腺癌标本cystatin M表达水平与乳腺癌病人的雌激素受体状态(ER)及孕激素状态(PR)无关.结论 Cystatin M是一种监测乳腺癌是否发生转移的一种可靠的指标.

The expression and clinical significance of cystatin M in breast cancer

Objective To investigate the expression of cystatin M in the breast tumor. Methods RT-PCR was used to measure the level of cystatin M mRNA in 52 samples of breast cancer and the adjacent normal breast tissues, which was analyzd for understanding its relationship with clinical and pathological parameters. Results The expression levels of cystatin M in the breast cancer tissue were not significantly different from those in the benign breast tumor and neighboring normal breast tissue. But cystatin M expression levels in the malignant cancer tissue with lymph nodes metastasis were significantly lower than those in the neighboring normal tissue and the malignant cancer tissue without lymph nodes metastasis. Cystatin M expression levels of breast cancer were not correlated with estrogen receptor or progestone receptor status. Conclusion The cystatin M is a marker that could be used to predict the metastasis of breast cancer.