汉族人血小板GPⅠbHPA-2基因多态性与冠心病血瘀证的相关性研究

目的 观察GPⅠbHPA-2（Ko^b／Ko^a）基因多态性，在北京河北地区汉族人中各基因型的分布频率，分析该多态性与冠心病、冠心病血瘀证易感性的相关性。方法 采用病例对照设计，筛选符合冠心病、血瘀证、健康人入选标准的110例冠心病血瘀证、102例冠心病非血瘀证患者及106例健康对照人群为研究对象，进行中医辨证分型、血瘀证计分，并记录冠脉造影病变支数，采用基因测序技术检测HPA-2基因多态性。结果 GPⅠbHPA-2a／2a、HPA-2a／2b、HPA-2b／2b各占90．9％，8．8％和0．3％，所有入选病例中仅有1例表达HPA-2b／2b型；冠心病组和健康对照组、冠心病血瘀证组和冠心病非血瘀证组的基因型构成，冠心病患者不同病变支数基因型构成，冠心病血瘀证各基因型患者的血瘀证计分，各组间比较均无显著性差异（P〉0．05）。以是否患冠心病为因变量的二分类Binary Logistic回归分析，校正年龄、性别、体重指数对冠心病的影响后，结果显示HPA-2多态位点基因型与冠心病发病无相关性。结论 GPⅠb的HPA-2多态位点不是汉族人冠心病、冠心病血瘀证的独立危险因素。

Research on the Association between the Platelet Membrane Glycoprotein I b Polymorphism and Blood-Stasis Syndrome of Coronary Heart Disease in Han people

Objective To learn the genotype distributions of HPA-2 （Kob/Ko^a） in Han people from Beijing and Hebei Province, and to study the association of the platelet glycoprotein Ⅰ b polymorphism with coronary heart disease （CHD）, blood-stasis syndrome of CHD. Methods One hundred and ten patients with blood-stasis syndrome of coronary heart disease, 102 patients with the non-blood stasis syndrome of coronary heart disease and 106 healthy controls were enrolled in this case-control study. Differentiation of symptoms and signs for the classification of syndrome, scores of blood-stasis syndrome, and the number of occlusive coronary artery were performed or measured in all subjects. The genotypes of HPA-2 were determined by DNA sequencing technology. Results The frequencies of HPA-2 polymorphism were respectively 90.9%, 8.8% and 0.3%. There was only one carrier of HPA-2b/2b in our study, and the frequencies of HPA-2 polymorphism had no difference in CHD and healthy controls. The prevalence of HPA-2 genetic variants differ neither in blood-stasis syndrome of CHD and blood-stasis syndrome of CHD nor in CHD patients of different numbers of occlusive coronary artery. The scores of blood-stasis syndrome had no difference between different genotypes. Results were statistically evaluated with Binary Logistic Regression, which showed the HPA-2 polymorphism had no association with CHD. Conclusions The HPA-2 polymorphism of GP Ⅰ b is not the risk factor of CHD and blood-stasis syndrome of CHD in Han people.