| 竹红菌甲素敏化S-180肿瘤细胞膜的光氧化反应及机理探讨 |
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| 引用本文： | 张力,贾弘禔,董苍玉.竹红菌甲素敏化S-180肿瘤细胞膜的光氧化反应及机理探讨[J].北京大学学报(医学版),1993(2). |
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| 作者姓名： | 张力 贾弘禔 董苍玉 |
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| 作者单位： | 北京医科大学生物化学教研室
(张力，贾弘禔)，北京医科大学生物化学教研室(董苍玉) |
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| 摘 要： | 本文研究了竹红菌甲素(简称甲素)对S-180肿瘤细胞膜的作用。结果表明,甲素结合光照可引起S-180肿瘤细胞膜蛋白质交联、脂质过氧化产物MDA含量增加。但在SDS-PAGE分析中观察到的高分子蛋白质交联带并非由MDA所引起。甲素同样可引起小牛胸腺组蛋白光氧化、发生分子交联,并可敏化色氨酸、酪氨酸和组氨酸的无氧化。
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| 关 键 词： | 竹红菌甲素 光敏反应 蛋白质交联 |
THE PHOTOOXIDATION OF S-180 CELL MEMBRANE SENSITIZED BY HYPOCRELLIN A AND ITS MECHANISM |
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| Zhang Li.THE PHOTOOXIDATION OF S-180 CELL MEMBRANE SENSITIZED BY HYPOCRELLIN A AND ITS MECHANISM[J].Journal of Peking University:Health Sciences,1993(2). |
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| Authors: | Zhang Li |
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| Institution: | Zhang Li Department of Biochemistry |
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| Abstract: | In the present study the oxidation of asoitic sarcoma 180 cell membrane photosensitized by Hypo-crellin A has been investigated. SDS-PAGE showed obviously increased molecular weights, suggestingprotein cross-links. A significant increase of MDA in membrane was observed after illumination whichindicated that lipid peroxidation occured in Hypocrellin A-sensitized photooxidation. In other experi-ment we demonstrated that protein cross-1inks were not induced directly by MDA. Hypocrellin A couldalso induce the chages of histidine, tyrosine and tryptophan in photooxidation. So we assume that theformation of protein cross-links may be mediated by photooxidized amino acids in proteins. |
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| Keywords: | Hypocrellin A Photosensitized Oxidation Protein cross-link |
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