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昆明山海棠片对小鼠淋巴细胞体外增殖活化影响的研究
引用本文:徐艳,郑永唐,何黎,万屏,农祥.昆明山海棠片对小鼠淋巴细胞体外增殖活化影响的研究[J].中药材,2008,31(4):557-561.
作者姓名:徐艳  郑永唐  何黎  万屏  农祥
作者单位:1. 昆明医学院第一附属医院皮肤科,云南昆明,650032
2. 中国科学院昆明动物研究所,云南昆明,650223
摘    要:目的:研究昆明山海棠片对小鼠脾淋巴细胞增殖、活化标志CD69和CD25及细胞因子IL-4及IFN-γ的影响。方法:无菌分离小鼠脾淋巴细胞,加入不同浓度的昆明山海棠溶液,MTT法检测刀豆蛋白A刺激的小鼠脾淋巴细胞增殖;流式细胞仪检测活化标志CD69及CD25;酶联免疫法检测细胞因子IL-4及IFN-γ。结果:昆明山海棠片对脾淋巴细胞增殖活化及分泌均有抑制作用。

关 键 词:昆明山海棠片  淋巴细胞增殖  淋巴细胞活化  免疫抑制  细胞因子  昆明山海棠片  小鼠脾淋巴细胞  细胞体外  增殖活化  影响  研究  Mouse  Tripterygium  hypoglaucum  Hutch  Study  in  vitro  抑制作用  结果  酶联免疫法检测  流式细胞仪检测  刺激  刀豆蛋白  溶液  浓度  菌分离  方法
文章编号:1001-4454(2008)04-0557-05
修稿时间:2007年8月28日

Study of Tripterygium hypoglaucum Hutch on Mouse's Splenic Lymphocyte Activation in vitro
XU Yan,ZHENG Yong-tang,HE Li,WAN Ping,NONG Xiang.Study of Tripterygium hypoglaucum Hutch on Mouse''''s Splenic Lymphocyte Activation in vitro[J].Jorunal of Chinese Medicinal Materials,2008,31(4):557-561.
Authors:XU Yan  ZHENG Yong-tang  HE Li  WAN Ping  NONG Xiang
Institution:Department of Dermatology, First Affiliated Hospital of Kunming College, Kunming 650032, China. xuyanfirst@163.com
Abstract:OBJECTIVE:To study the effects of Tripterygium hypoglaucum Hutch (THH) on the mouse's splenic lymphocyte proliferation, activation mark such as CD69 and CD25, cytokine such as IL-4 and gamma-IFN. METHODS: Separating the mouse's splenic lymphocyte sterilitily and adding THH of different density. The proliferation of the ConA-stimulated splenic lymphocyte was measured with MTT; the activation marks such as CD69 and CD25 were measured with flow cytometer; cytokines such as IL-4 and gamma-IFN were measured with euzymelinked immunosorbent assay. RESULTS: THH had inhibitory action on both proliferation and activation of splenic lymphocyte.
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