| 昆明山海棠片对小鼠淋巴细胞体外增殖活化影响的研究 |
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| 引用本文： | 徐艳,郑永唐,何黎,万屏,农祥.昆明山海棠片对小鼠淋巴细胞体外增殖活化影响的研究[J].中药材,2008,31(4):557-561. |
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| 作者姓名： | 徐艳 郑永唐 何黎 万屏 农祥 |
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| 作者单位： | 1. 昆明医学院第一附属医院皮肤科,云南昆明,650032
2. 中国科学院昆明动物研究所,云南昆明,650223 |
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| 摘 要： | 目的：研究昆明山海棠片对小鼠脾淋巴细胞增殖、活化标志CD69和CD25及细胞因子IL-4及IFN-γ的影响。方法：无菌分离小鼠脾淋巴细胞,加入不同浓度的昆明山海棠溶液,MTT法检测刀豆蛋白A刺激的小鼠脾淋巴细胞增殖;流式细胞仪检测活化标志CD69及CD25;酶联免疫法检测细胞因子IL-4及IFN-γ。结果：昆明山海棠片对脾淋巴细胞增殖活化及分泌均有抑制作用。
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| 关 键 词： | 昆明山海棠片 淋巴细胞增殖 淋巴细胞活化 免疫抑制 细胞因子 昆明山海棠片 小鼠脾淋巴细胞 细胞体外 增殖活化 影响 研究 Mouse Tripterygium hypoglaucum Hutch Study in vitro 抑制作用 结果 酶联免疫法检测 流式细胞仪检测 刺激 刀豆蛋白 溶液 浓度 菌分离 方法 |
| 文章编号： | 1001-4454(2008)04-0557-05 |
| 修稿时间： | 2007年8月28日 |
Study of Tripterygium hypoglaucum Hutch on Mouse's Splenic Lymphocyte Activation in vitro |
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| XU Yan,ZHENG Yong-tang,HE Li,WAN Ping,NONG Xiang.Study of Tripterygium hypoglaucum Hutch on Mouse''''s Splenic Lymphocyte Activation in vitro[J].Jorunal of Chinese Medicinal Materials,2008,31(4):557-561. |
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| Authors: | XU Yan ZHENG Yong-tang HE Li WAN Ping NONG Xiang |
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| Institution: | Department of Dermatology, First Affiliated Hospital of Kunming College, Kunming 650032, China. xuyanfirst@163.com |
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| Abstract: | OBJECTIVE:To study the effects of Tripterygium hypoglaucum Hutch (THH) on the mouse's splenic lymphocyte proliferation, activation mark such as CD69 and CD25, cytokine such as IL-4 and gamma-IFN. METHODS: Separating the mouse's splenic lymphocyte sterilitily and adding THH of different density. The proliferation of the ConA-stimulated splenic lymphocyte was measured with MTT; the activation marks such as CD69 and CD25 were measured with flow cytometer; cytokines such as IL-4 and gamma-IFN were measured with euzymelinked immunosorbent assay. RESULTS: THH had inhibitory action on both proliferation and activation of splenic lymphocyte. |
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