益肾活血胶囊对高同型半胱氨酸致兔血管平滑肌细胞核因子活化及血管细胞黏附分子表达的影响

目的：探讨益肾活血胶囊提取液（YSHXC）对高同型半胱氨酸（HCY）致兔血管平滑肌细胞（VSMCs）核因子-κB（NF—κB）活化及血管细胞黏附分子-1（VCAM—1）表达的影响。方法：以HCY诱导体外培养的兔主动脉平滑肌细胞建立细胞增殖模型。实验分为正常组、HCY组、HCY＋YSHXC（小、中、大）剂量组。通过细胞计数观察VSMCs的增殖情况，同时采用免疫组化和RT—PCR技术分别检测NF—κB、vCAM—1的蛋白及mRNA表达。结果：HCY诱导后可使NF—κB活化、VCAM—1的表达增强；而YSHXC可从蛋白和mRNA水平明显抑制其表达（P〈0．01），并呈现剂量依赖关系，这在各实验组细胞数目也有相应的体现。结论：YSHXC能明显抑制HCY诱导的NF—κB活化及VCAM—1的表达，减少VSMCs的增殖和向内皮的迁移，可能对延缓动脉粥样硬化（AS）的发生发展起到一定作用。

Effects of "Yishen Huoxue Capsule" on NF-kB and VCAM-1 of Rabbits' VSMCs Induced by HCY

Objective： To investigate the effects of ＂Yishen Huoxue Capsule＂（YSHXC）on NF-κB and VCAM-1 of rabbits＇ VSMCs induced by HCY. Methods： Cultured cells proliferation model of rabbits＇ VSMCs were established with HCY in vitro. Five groups were set up as follows： blank group, model group, low-dose YSHXC group, middle-dose YSHXC group and high-dose YSHXC group. The VSMCs proliferation was determined by cell counting. The expressions of protein and mRNA of NF-κB and VCAM-1 were detected with immunohistocbemistry and RT-PCR respectively. Results： HCY induced the activating of NF-κB as well as the expression of VCAM-1. YSHXC significantly reduced the expression of protein and mRNA of NF-κB and VCAM-1 in a dose-dependent manner, which can also be reflected from the cells counting. Conclusion： YSHXC can significantly inhibit the activation of NF-κB and the expression of VCAM-1 induced by HCY, reduce the VSMCs＇ proliferation and its migration to the endothelium, thus may delay the occurrence and development of artberosclerosis（AS）.