顺铂和阿霉素对K562细胞周期及凋亡影响的研究

目的：观察化疗药物顺铂（DDP）和阿霉素（ADM）对细胞周期及凋亡的影响。方法： 以不同剂量的药物处理细胞，用流式细胞仪检测处理后不同时间细胞周期变化。结果： 10 μmol/L的DDP作用36 h及1.36 μmol/L的ADM作用48 h,K562细胞以S期阻滞最明显。20 μmol/L的DDP作用24 h出现G1期阻滞，0.17 μmol/L的ADM作用下以G2/M期阻滞为主。在10 μmol/L的DDP 和1.36 μmol/L的ADM作用下，随作用时间延长，阻滞的细胞释放，部分进入凋亡导致凋亡增加。结论： 不同剂量的DDP或ADM作用不同时间导致不同的细胞周期检测点激活，从而使细胞周期变化呈现不同的规律。

Influence of DDP and ADM on cell cycle and apoptosis in K562 cells

AIM: To investigate the effect of DDP and ADM on cell cycle in leukemia cell line K562. METHODS: The change of cell cycle was observed by means of flow cytometry after different interval in which the K562 cells were treated with DDP and ADM. RESULTS: K562 cells attested at S phase after treated 36 hours with DDP at concentration of 10 μmol/L or 48 h with ADM at concentration of 1.36 μmol/L. K562 cells attested at G1 phase after treated 24 h with DDP at concentration of 20 μmol/L or at G2/M phase after treated with ADM at concentration of 0.17 μmol/L. CONCLUSIONS: K562 cells showed different patterns to change the cell cycle induced by DDP and ADM with different concentrations at different time, which reflected that different checkpoints of cell cycle were activated at different conditions.