人白血病细胞周期分布的流式细胞术分析

应用流式细胞术(Flow cytometry,FCM)测定不同类型白血病和骨髓增生异常症(MDS)患者骨髓及外周血细胞周期分布。并对其增殖特性和生物学意义进行讨论和分析。结果提示,白血病骨髓细胞的增殖活性明显低于正常水平,并显示增殖状态的异质性。骨髓增生异常症患者骨髓细胞周期分布与临床分型和预后有关。

Flow cytometric analysis of cell-cYcle distribution in human leukemias

The cell cycle distribution of bone marrow and/or blood samples from 86 patients with leukemias and myelodysplastic syndromes and 28 healthy controls were detected and analyzed with DNA-flow cytometry. The fraction of proliferative phase cells (S + G_2M) of the marrow in the majoraty of patients with ALL, ANLL, CML and CLL was Lower than in the normal controls (P<0.001 or 0.05), and the cell proliferation in acute leukemia individuals showed considerable variation.In acute leukemia, the percentage of S + G_2M in the marrow was higher than in the blood. No relationship was observed between S + G_2M% and morphologically identifiable blast cells in the original marrow and blood samples. The cell cycle dis tribution of marrow in myelodysplastic syndromes was related to the clinical categories and prognosis. The mean percentage of S + G2M in RA and RAS was close to the normals but that in RAEB and RAEB-T was similar to that in acute leukemia.