Correlation between biliaryα 1-acid glycoprotein concentration and cholesterol crystal nucleation time in gallstone disease |
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Authors: | Hannu Nuutinen Stefano Ginanni Corradini Dieter Jüngst Volker Lange Masato Abei Jörg Schwarzendrube Carlumandarlo Williams R. Thomas Holzbach |
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Affiliation: | 1. From the Gastrointestinal Research Unit, Research Institute, and Departments of Gastroenterology and General Surgery, Cleveland Clinic Foundation, 9500 Euclid Ave., 44195-5218, Cleveland, Ohio 2. Departments of Medicine II and Surgery, Klinikum Grosshadern, Ludwig Maximilians-University, Munich, 8000, Munich 70, Germany
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Abstract: | A biliary form of theα 1-acid glycoprotein (AAG) promotes cholesterol crystallization in the lower-molecular-weight, concanavalin A-bound fraction of gallbladder bile. In addition, bile AAG concentration is higher in cholesterol gallstone patients with multiple stones than in control patients without gallstone disease. In this study we sought to determine whether the increased biliary concentration of AAG in cholesterol gallstone patients is accompanied by a more rapid nucleation time in patients with multiple stones. AAG concentration in native biles was measured by ELISA. Nucleation time was measured using a standard microscopy method. The concentration of biliary AAG was then related to nucleation time in biles from the same patients. Nucleation times were significantly shorter (≤5 days) in cholesterol gallstone patients with raised AAG concentrations (P<0.03). There was a significant (P=0.004) negative correlation (r=?0.53) between nucleation time and the AAG concentration in cholesterol gallstone patients with multiple stones. The concentration of biliary AAG appears to exert an important influence on the speed of cholesterol nucleation in bile in many patients with cholesterol gallstone disease. |
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