肺癌患者支气管镜活检标本端粒酶活性的测定

目的 验证肺癌中端粒酶活性的存在,探寻支气管镜活检标本的端粒酶活性测定在肺癌诊断中的价值。方法 以端粒重复扩增技术（ＴＲＡＰ）分析手术标本及支气管镜活检标本的细胞提取液中端粒酶的活性,ＴＲＡＰ分析的结果与病理检查结果进行比较,结果 １０例肺癌手术标本中均检测到端粒酶活性。１０例手术获得的正常肺组织中没有检测到端粒酶活性。１１例肺癌支气管镜活检标本中有８例检测垤端粒酶活性。３例肺良性病变支气管镜活检

A preliminary study on telomerase activity in lung cancer patients' bronchoscopically biopsied specimens.

Objective To detect the levels of telomerase activity in lung cancer tissues and explore the value of telomerase activity analysis in specimens obtained by bronchoscopic biopsy in the diagnosis of lung cancer.Methods Extracts of cells in surgically resected specimens and bronchoscopically biopsied specimens were analyzed for telomerase activity by the telomeric repeat amplification protocol (TRAP) assay. The results of TRAP assays were compared with those of pathological examination. Results Telomerase activity was positive in surgically resected specimens from 10 of 10 lung cancer tissues and from 0 of 10 normal lung tissues. Telomerase activity was positive in bronchoscopically biopsied specimens from 8 of 11 lung cancer tissues, from 1 of 3 benign lung disease tissues, and from 3 of 12 normal bronchial mucosas. Conclusion Lung cancer tissues have detectable telomerase activity. Detection of telomerase activity in bronchoscopic biopsied specimens may be helpful in the diagnosis of lung cancer.