重组胰高血糖素样多肽2对烧伤大鼠肠黏膜的保护作用

目的验证重组胰高血糖素样多肽2（GLP-2）对严重烧伤大鼠的肠道保护作用。方法将SD大鼠随机分为正常对照组；烧伤对照组；重组GLP-2治疗组（重组治疗组），烧伤后4h皮下注射重组GLP-2，100nmol·kg^-1·d^-1；化学合成GLP-2治疗组（合成治疗组），烧伤4h后皮下注射合成GLP-2，剂量同上。每组6只大鼠。伤后第7天检测各致伤组大鼠肠黏膜通透性、肠黏膜湿质量与肠段及躯壳质量比、肠黏膜蛋白含量以及观察肠道组织病理学变化，正常对照组观察指标相同。结果重组治疗组及合成治疗组与烧伤对照组（0．350±0．040）mg／m1]比较，大鼠肠黏膜通透性明显降低（P〈0．01），分别为（0．250±0．026）、（0．243±O．008）mg／ml；肠黏膜湿质量与躯壳质量比及肠黏膜蛋白含量明显增加，重组治疗组大鼠肠黏膜蛋白含量为（57．9±2．8）mg／g，高于合成治疗组（48．9±4．1）mg／g。与正常对照组比较，各致伤组大鼠伤后第7天肠黏膜绒毛明显变短脱落、排列紊乱、基底层变薄。重组治疗组损伤较烧伤对照组有所减轻，与合成治疗组大鼠无明显区别。结论重组GLp02与合成GLP-2，能减轻烧伤大鼠肠道损伤，具有明显的肠道保护作用。

The protective effect of recombinant glucagons like peptide-2 on intestinal mucosa of burn rats

OBJECTIVE: To investigate the protective effect of recombinant glucagons like peptide-2 (GLP-2) on intestinal mucosa of rats with severe burns. METHODS: SD rats of either sex were randomly divided into 4 groups: normal control (N, n = 6), burn control group (C, n = 6), recombinant GLP-2 group (Gr, n = 6, with subcutaneous injection of 100 nmol x kg(-1) x d(-1) recombinant GLP-2 at 4 post-burn hours (PBH) and synthesized GLP-2 group (G, n = 6, with subcutaneous injection of 100 nmol x kg(-1) x d(-1) synthesized GLP-2 at 4 PBH). Except the normal control group, all animals in the other groups received a 30% TBSA third degree burns, the rats were sacrificed on 7 postburn days (PBD) and the following indexes were determined: pathological examination of intestinal mucosa, mucosa permeability of intestinal mucosa, the ratio of mucosa wet weight and bowel mass or carcase weight, and the protein content of intestinal mucosa. RESULTS: Compared with that in burn group (0.350 +/- 0.040) mg/ml], the mucosa permeability significantly decreased in Gr (0.250 +/- 0.026) mg/ml and G (0.243 +/- 0.008) mg/ml groups, while the ratio of mucosa wet weight and carcase weight, the protein content of intestinal mucosa were significantly increased. In additon, the content of intestinal mucosal protein in Gr group (57.9 +/- 2.8) mg/g wet weight] was higher than that in G group (48.9 +/- 4.1) mg/g wet weight]. In contrast to normal controls, the villi of intestinal mucosa in rats on 7 PBD were obviously shortened and exfoliated, with deranged disposition and thinned basal membrane. No obvious difference of the injury was observed between Gr and G groups, and the injury was milder when compared with burn group. CONCLUSION: Recombinant GLP-2, as well as synthesized GLP-2, exhibits obvious protective effect on intestinal mucosa in alleviating injury to intestinal mucosa in burn rats.