A quantitative system for studying phagocytosis in pigment epithelium tissue culture. |
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| Authors: | A I Goldman P J O'Brien E Masterson P Israel P Teirstein G Chader |
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| Institution: | Laboratory of Vision Research, National Eye Institute, National Institutes of Health, U.S. Department of Health, Education and Welfare, Bethesda, Md 20014, U.S.A. |
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| Abstract: | A quantitative assay for phagocytosis has been described using a heterologous system of chick pigment epithelial culture and radiolabeled bovine rod outer segment suspensions. Electron microscopy was used to verify results obtained in radiolabeled uptake studies. Freezing and mild shearing of the outer segment suspensions do not significantly inhibit phagocytosis. Outer segments radiolabeled with 3H]choline and 14C]mannose showed similar kinetics of phagocytosis. Maximum uptake was between 1 and 2 hr of incubation and approached 1% of the total applied radioactivity for mannose and 2% for choline. |
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| Keywords: | phagocytosis pigment epithelium cell culture electron microscopy mathematical models histochemistry microvilli outer segments |
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