没食子有效部位抗溃疡性结肠炎的实验研究

目的研究没食子有效部位对溃疡性结肠炎(UC)模型大鼠的治疗作用。方法采用三硝基苯磺酸和乙醇复合方法制备大鼠UC模型,经病理切片证实造模成功后,随机分为没食子有效部位高、低剂量组和模型组、柳氮磺吡啶组。灌胃给药20 d后,评价大鼠结肠黏膜损伤程度(CMDI);检测体重、肠重指数和免疫器官重量指数的变化;检测大鼠结肠组织中超氧化物歧化酶(SOD)、髓过氧化物酶(MPO)活性。结果没食子有效部位高剂量组溃疡均完全愈合,与模型组比较,没食子有效部位高、低剂量组结肠组织中SOD显著增高(P0.01),MPO显著降低(P0.01)。结论没食子有效部位对大鼠UC有明显的治疗作用。

Experimental Study of Effective Fractions of Galla Turcica in Treating Ulcerative Colitis in Rats

Objective To observe the effect of Gallo tannins in treating experimental ulcerative colitis （UC） in rats. Methods The UC model rats was made by TNBS and ethanol. After confirmed by the pathological section, the model rats were randomly divided into four groups： two Gallo tannins groups （treated with Gallo tannins of different concentrations）, model group and SASP treatment group. Meanwhile a normal control group was designed. Different groups were given different treatment respectively. Twenty days later, the colon mucosal damage index （CMDI） was evaluated. Intestines weight index, body weight, SOD, MPO of all the groups were assayed. Results The groups of Gallo tannins with high dose can relieve the inflammatory reaction and colonic injury. The level of MPO in high and low dose of GaUo tannins groups was higher than that in model group （P〈0.01）, and the tissue level of SOD markedly decreased （P〈0.01）. Conclusion Gallo tannins is effective in treating UC rats.