荆半夏组织培养及快速繁殖的研究

目的建立荆半夏快速繁殖体系,为抢救性开发荆半夏野生资源提供有力手段。方法用MS+6BA2.0mg·L-1+IBA0.5mg·L-1培养基诱导及分化培养;用MS+6BA0.2mg·L-1+IBA1.0mg·L-1培养基作生根培养。结果诱导培养2周后形成大量愈伤组织,继代培养3周后分化出大量丛生芽。生根培养2周后形成完整植株。结论通过组培快繁荆半夏的繁殖系数由自然栽培时的每年1~2倍提高到每月15倍左右。

Research of tissue culture and rapid propagation of Pinellia ternate from Jingzhou

Objective In order to explore a effective method for protection the wild Pinellia ternate in Jingzhou. The rapid propagation system of Pinellia ternate was established.Methods A mixture of MS+6BA 2.0 mg·L~ -1 +IBA 0.5 mg·L~ -1 was used as induce and polarized culture medium. And medium of MS+6BA 0.2 mg·L~ -1 +IBA1.0 mg·L~ -1 was used as the rootage culture.Results A mass of calli was formed in two weeks, and a mass of caespitose bud was induced after sequentially generational cultivation in three weeks. Plant was integrated after two weeks rootage cultivation.Conclusion The quotiety of propagation of Pinellas ternate Jingzhou increases from about 1~2 times a year by normal method to 15 times a month by our method.