| 孕中期羊水干细胞的生物学特性的研究 |
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| 引用本文： | 常颖,高垚,李欢,王欢欢,刘松岩. 孕中期羊水干细胞的生物学特性的研究[J]. 中国实验诊断学, 2012, 16(6): 958-961 |
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| 作者姓名： | 常颖 高垚 李欢 王欢欢 刘松岩 |
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| 作者单位： | 1. 吉林大学中日联谊医院,神经内一科,吉林,长春130033
2. 长春市中心医院,神经内科,吉林,长春130051 |
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| 基金项目： | 吉林省科技厅资助项目（200705268） |
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| 摘 要： | 目的对孕中期羊水标本进行体外分离、培养、扩增,研究孕中期羊水来源干细胞(AFC)的生长特性、细胞表面标记。方法采用贴壁法体外分离孕中期羊水来源干细胞,绘制细胞生长曲线;流式细胞仪检测细胞表面标记。结果原代培养细胞生长潜伏期4-6d,对数增殖期7-13d,生长平台期14-18d;传代培养细胞生长潜伏期1-2d,对数增长期3-6d,生长平台期7-10d。AFCs均一表达CD44,不表达CD45。结论从人孕中期羊水中成功分离具有干细胞性质的细胞群,在体外可迅速扩增。
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| 关 键 词： | 羊水 干细胞 流式细胞术 细胞分离 扩增 |
Research on bioiogicfal features of human amniotic fluid-derived stem cells in second-trimester |
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| Affiliation: | CHANG Ying, GAO Yao,Ll Huan, et al. (1. Department of Neurology China-Japan Union Hospital, Jilin University, Changchun 130033, China ; 2. Department of Neurology, Changchun Central Hospital, Changchun 130051, China) |
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| Abstract: | Objective Human amniotic fluid samples in second trimester. To study the growth pattern and surface markers of human amniotic fluid derived stem cells in second-trimester. Methods The growth curves of the AFCs were drawn. The flow cytometry was used to detect surface antigenic markers. Results The latent phase of the primary culture cells was 4 6days,followed by 7 13 clays of logarithmical proliferation; the plateau began at day 14 18. The sub cultures showed 1-2 days of latent phase, followed by 3-6 days of logarithmical proliferation, then entered the plateau （days） 7 10. The AFCs expressed positive CD44 unanimously,but don＇t expressed CD45. Conclusion Experiment has successfully isolated stem cells from second trimester amniotie fluid. These stem cells can he expanded in vitro rapidly. |
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| Keywords: | Amniotic fluid Mesenchymal stem cells Flow cytometry ~ Cell separation Amplification |
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