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| 双抗体夹心ELISA检测人胎儿血红蛋白体系的建立 | |
| 引用本文： | 李雪丽,文李艳,冯善伟,钟梅,刘艳君,富宁. 双抗体夹心ELISA检测人胎儿血红蛋白体系的建立[J]. 中国实验诊断学, 2012, 16(6): 997-1000 |
| 作者姓名： | 李雪丽 文李艳 冯善伟 钟梅 刘艳君 富宁 |
| 作者单位： | 1. 南方医科大学基础医学院,免疫教研室,广东,广州510515 2. 广州市人口和计划生育科学研究所,广东,广州510410 3. 南方医科大学,南方医院,广东,广州510515 |
| 基金项目： | 广东省科技项目20078031512001 |
| 摘 要： | 目的建立一种定量检测人胎儿血红蛋白(HbF)的双抗体夹心ELISA方法。方法用抗人HbF单抗作为捕获抗体,加入待测抗原,检测抗体为兔抗人HbF多克隆抗体,最后加入辣根过氧化物酶标记的羊抗兔IgG。结果本检测体系灵敏度约为0.039μg/ml,测量范围0.039-24.66μg/ml,特异性强,重复性好,100份血标本的检测结果与高效液相色谱法结果符合率高。结论建立了一种可用于检测人外周血HbF的双抗体夹心ELISA方法,有望为临床上诊断某些与HbF有关的疾病提供帮助。 |
| 关 键 词： | 胎儿血红蛋白 双抗体夹心ELISA 多克隆抗体 |
Establishment of a sandwich-ELISA system for determination of human fetal hemoglobin |
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| Affiliation: | LI Xue-li,WEN Li-yan,FENG Shan-wei,et al. (1. Department of Immunology, School of Bacsic Medical Sciences, Southern Medical Uni- versity, Guangzhou 510515, China;2. Institute for Population and Family Planning of Guangzhou City, Guangzhou 510410 ,China) |
| Abstract: | Objective To establish a sandwich ELISA for the determination of fetal hemoglobin （HbF） in human blood. Methods An anti-HbF monoclonal antibody was coated on polystyrene microtiter plates followed by blocking with 5% defat milk,then samples for detection were added to the wells,followed by anti-HbF polyclonal antibody prepared from rabbit;at last＇, addition of HRP-goat anti-rabbit IgG and conventionhl color development were performed. Resuits This sandwich ELISA system can determine HbF specifically,the sensitivity for HbF is 0. 039μg/ml,the range of detection is 0. 039-24.66μg/ml. Conclusion This HbF ELISAis applicable for research and observation in clinical. |
| Keywords: | Human Fetal hemoglobin ssandwich ELISA polychonal antibody |
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