海水浸泡对失血性休克大鼠心肌和肝细胞线粒体功能的影响

目的：研究海水浸泡对失血性休克大鼠心肌和肝细胞线粒体功能的影响。方法：采用雄性Wistar大鼠24只,分为正常对照组,平原休克组和海水休克组,每组8只。测定血液动力学及心肌和肝细胞线粒H^ -ATP酶、琥珀酸脱氢酶（SDH）,Ca^2 -Mg^2 -ATP酶,质子转运及线粒体总钙的变化。结果：海水浸泡失血性休克大鼠血液动力学,心肌和肝细胞线粒体H^ -ATP酶,SDH,Ca^2 -Mg^2 -ATP酶活性均显著低于正常对照组和平原休克组,线粒体总钙含量显著高于平均体克组,海水浸泡失血性休克亚线粒体质子跨膜转运能力与平原休克组比较,差异无显著性意义。结论：海水浸泡失血性休克大鼠心肌和肝细胞线粒体酶活性显著下降,线粒体钙含量升高,伤情显著加重。

Effect of sea water immersing on the functions of mitochondria of myocardium and hepatocyte in hemorrhagic shock rats

Objective To investigate effcet of sea water immersing on the functions of mitochondria of myocardium and hepatocyte in hemorrhagic shock rats. Methods Twenty four male rats were divided into 3 groups: control group ( n =8); hemorrhagic shock group on land (Group A, n =8); sea water immersing hemorrhagic shock group (Group B, n =8). The activity of H ATPase, succinodehydrogenase (SDH) and Ca 2 Mg 2 ATPase,calcium contents of myocardium, and hepatocyte mitochondria were measured. The changes of proton translocation across the inner mitochondrial membrane (submitochondria particle membrane) were analyzed. Results Hemodynamic indexes and activities of H ATPase, SDH and Ca 2 Mg 2 ATPase of mitochondria in Group B were significantly lower than that in control group and Group A ( P <0.05).The calcium contents of mitochondria of myocardium and hepatocyte in Group B were significantly elevated compared with control group and Group A ( P <0.05). There were no significant differences between Group A and Group B about proton translocation. Conclusions Mitochondria enzyme activities are lowered, but calcium contents in tissues and mitochondria elevated. The shock in Group B is more serious than that of other 2 groups.