结核性和恶性胸膜炎患者VEGF的水平及意义

目的 通过检测结核性胸膜炎及恶性胸膜炎患者血清和胸水中血管内皮生长因子（VEGF）含量,分析VEGF在两组患者血清、胸水中的差异,探讨VEGF在二者中的意义和诊断价值.方法 对确诊结核性胸膜炎和恶性胸膜炎各30例的患者在同一日留取胸水标本10 ml及静脉血5 ml,采用双抗体夹心酶联免疫吸附试验检测患者胸水及血清中VEGF水平,分析其差异及相关性.结果 结核组血清和胸水VEGF检测值分别为（45.33±18.33） ng/L、（62.73±24.65） ng/L;恶性组血清和胸水VEGF检测值分别为（66.00±29.83） ng/L、（95.54±42.11） ng/L;恶性组血清及胸水中VEGF含量均高于结核组（t值分别为3.9、5.2,P值均＜0.05）.VEGF在两组的血清和胸水中均呈正相关性（r值分别为0.53、0.38,P值均＜0.05）.结论 在结核性胸膜炎及恶性胸膜炎患者血清、胸水中VEGF水平有差异,恶性高于结核性;两组患者胸水中VEGF含量均高于血清,胸水VEGF含量随着血清VEGF含量增高而增高.检测胸腔积液患者血清和胸水中VEGF含量对结核性胸膜炎和恶性胸膜炎的诊断和鉴别诊断有一定的价值.

VEGF level and significance of tuberculous pleurisy and malignant pleurisy

Objective To analyse the difference and explore the diagnosis significance of vascular endothelial growth factor （VEGF） in serum and pleural fluid of patients with tuberculous pleurisy and malignant pleurisy by measuring the concentrations of VEGF in serum and pleural fluid.Methods 10 ml pleural fluid and 5 ml blood as specimen from patients with tuberculous pleurisy and malignant pleurisy （each 30 cases） were collected on the same day.The VEGF concentrations of serum and pleural fluid were measured by the enzyme-linked immunosorbent assay.The difference and correlation were analysed.Results In tuberculous pleurisy group,the VEGF levels were （45.33 ± 18.33） ng/L in serum and （62.73± 24.65） ng/L in pleural fluid.In malignant pleurisy group,the VEGF levels were （66.00±29.83） ng/L in serum and （95.54± 42.11） ng/L in pleural fluid.The VEGF levels of serum and pleural fluid in malignant pleurisy group were higher than those in tuberculous pleurisy group （t =3.9,5.2,respectively,all P ＜0.05）.VEGF levels in serum and pleural fluid in the two groups showed positive correlation （r =0.53,0.38,respectively,all P ＜0.05）.Conclusions The levels of VEGF in serum and pleural fluid are different between patients with tuberculous pleurisy and patients with malignant pleurisy.The serum and pleural fluid VEGF levels in patients with malignant pleurisy are higher than those in patients with tuberculous pleurisy.VEGF level of pleural fluid is higher than that of serum in the two groups.Detection of VEGF in serum and pleural fluid has a certain value in diagnosis and differential diagnosis of patients with tuberculous pleurisy and malignant pleurisy.