类风湿关节炎患者血清GPI检测及其诊断价值的研究 |
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引用本文: | 沈宝岩,黄建军. 类风湿关节炎患者血清GPI检测及其诊断价值的研究[J]. 国际检验医学杂志, 2014, 35(4): 396-398 |
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作者姓名: | 沈宝岩 黄建军 |
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作者单位: | 沈宝岩 (上海交通大学医学院附属新华医院崇明分院检验科,上海崇明,202150); 黄建军 (皖南医学院弋矶山医院检验科,安徽芜湖,241001); |
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基金项目: | 安徽省教育厅高校自然科学基金资助项目(项目编号:KJ2013B316) |
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摘 要: | 目的探讨血清葡萄糖-6-磷酸异构酶(GPI)水平与类风湿关节炎(RA)发病的相关性及其对RA诊断的价值。方法采用ELISA法检测68例RA患者、69例非RA自身免疫性疾病患者和77例健康体检者血清GPI水平,常规检测RA患者类风湿因子(RF)、红细胞沉降率(ESR)、C反应蛋白(CRP)和抗CCP抗体水平。结果RA患者血清GPI水平为(7.01±8.03)mg/L,非RA自身免疫性疾病患者为(O.30士0.57)mg/L,健康体检者为(O.22±0.65)mg/L,RA患者血清GPI浓度显著高于非RA自身免疫性疾病患者和健康体检者(P〈0.001)。活动性RA患者和非活动性RA患者血清GPI水平比较,差异有统计学意义(P〈0.01)。以0.332mg/L为临界值,GPI检测用于RA诊断的灵敏度和特异度分别为70.6%和92.2%,ROC曲线下面积为0.901;GPI水平与RF、ESR、CRP及抗CCP抗体水平呈正关,相关系数分别为0.645、0.480、0.389和0.623(均P〈0.05)。结论RA患者血清GPI水平显著升高,GPI有可能成为诊断RA及判断RA活动性的一个新指标。
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关 键 词: | 关节炎 类风湿 葡萄糖-6-磷酸异构酶 诊断 |
Serum glucose-6-phosphate isomerase measurement and its diagnostic value on rheumatoid arthritis |
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Affiliation: | Shen Baoyan, Huang J ianjun (1 Department of Laboratory Medicine, Xinhua Hospital Chongming Branch Affiliated to Shanghai J iaotong University School of Medicine, Shanghai 202150, China ; 2 Department of Laboratory Medicine,Yijishan Hospital of Wannan Medical College ,Wuhu ,Anhui 241001 ,China) |
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Abstract: | Objective To detect the serum level of glucose-6-phosphate isomerase (GPI) and evaluate the diagnostic value on rheumatoid arthritis (RA). Methods The concentration of serum GPIin 68 patients with RA,69 non-RA patients with other auto- immune diseases and 77 normal controls were assayed by ELISA. The level of RF,CRP,anti-CCP antibody and ESR were also as- sayed in RA patients. Results GPI serum level were (7.01±8.03)mg/L in RA patients, (0. 304-0.57)mg/L in non-RA patients with other autoimmune diseases and (0.22 ±0.65)mg/L in control group. The level of GPI was statistically higher in RA patients than the other two groups (P~0.05), and it was also statistically higher in active RA patients than inactive RA patients. When 0. 332 mg/L was regarded as cut-off value,the sensitivity and specificity of GPI for RA were 70.6% and 92.2% respectively,and the area under the ROC curve was 0. 901. Spearman rank correlation analysis showed the level of serum GPI positively correlated with ESR,CRP and anti-CCP antibody,with correlation coefficients of 0. 480,0. 389 and 0. 623 respectively. Conclusion Serum lev- el of GPI increases significantly in RA patients and it could be used as a new indicator for RA diagnosis and activity judgment. |
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Keywords: | arthritis rheumatoid glucose-6-phosphate isomerase diagnosis |
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