TLR2对曲霉感染支气管上皮细胞时Dectin-1表达的影响

目的 探讨烟曲霉感染人支气管上皮（HBE）细胞时Toll样受体2（TLR2）对树突状细胞植物血凝素-1 （Dectin-1）表达的影响.方法 建立烟曲霉感染HBE细胞模型,通过PCR、Western blot方法分别在mRNA、蛋白水平检测Dectin-1和TLR2的表达情况;沉默TLR2,复制感染模型,应用Western blot及流式细胞技术评价Dectin-1的表达变化.结果 ①烟曲霉感染后HBE细胞TLR2表达水平虽然有所升高,并在感染18h达到峰值,但与静息状态表达水平的差异无统计学意义（P＞0.05）.②沉默TLR2,在烟曲霉感染HBE细胞后18 h时Dectiin1的蛋白表达受到显著抑制（P＜0.05）.结论 HBE细胞静息状态下能够表达TLR2,在烟曲霉感染早期,随着时间的延长其表达有逐步增高的趋势;HBE细胞启动Dectin-1的表达可能是建立在TLR2对烟曲霉早期识别的基础上,通过下游信号通路的激活来实现的。

Role of TLR2 in regulating Dectin-1 expression in Aspergillus fumigatus infected HBE cell line

Objective To investigate the effect of Toll like receptor 2 （TLR2） on Dectin-1 expression in HBE cells resulted from Aspergillus fumigatus stimulation.Methods The infection model in vitro was established via HBE cells exposed to Aspergillus fumigatus,and the mRNA and protein expressions of Dectin-1 and TLR2 were respectively detected by PCR and Westen blot.To explore the effect,TLR2 was silenced by siRNA and the infection model was copied.Then,the total expression of Dectin-1 was detected by Western blot and its expression on cell membrance was observed through the flow cytometry.Results TLR2 expression in HBE cells demonstrated an increasing trend after Aspergillus fumigatus stimulation,and reached a peak after 18 hours.However,the expression was not significantly different from the basal expression.Notably,the expression of Dectin-1 both in whole cell and on cell membrance was inhibited after 18 hours when TLR2 was silenced.Conclusions Our findings reveal an increasing expression of TLR2 after HBE cells exposed to Aspergillus fumigatus.And the expression of Dectin 1 in HBE cells triggered is probablely based on TLR2＇s early recoganization of Aspergillus fumigatus which activates the downstream signal transduction pathyway.