慢性阻塞性肺疾病患者呼出气冷凝液中髓过氧化物酶的检测及其意义

目的 检测COPD患者呼出气冷凝液（EBC）中髓过氧化物酶（MPO）浓度,探讨MPO与COPD发病发展的关系.方法 纳入49例COPD患者、18例健康体检者作为研究对象,采用EcoscrecnEBC收集仪采集标本,以酶免疫法测定EBC中MPO浓度.结果 COPD组EBC中MPO浓度显著高于健康对照组[（35.68±0.32）μg/L vs （11.72±0.34）μg/L,P＜0.05];COPD组EBC中MPO浓度与患者FEV1％ pred、呼气流量峰值呈正相关（相关系数r分别为0.418、0.327,P值均＜0.05）.结论 EBC中的MPO浓度能监测肺组织局部的炎症和氧化应激程度,能反映COPD患者病情严重程度,有助于发现COPD的病情变化,是一种较好的评价COPD病情的生物学指标.

Clinical significance of myeloperoxidase in exhaled breath condensate of patients with COPD

Objective To explore the clinical significance of myeloperoxidase（MPO） in exhaled breath condensate （EBC） in chronic obstructive pulmonary disease （COPD）.Methods Forty-nine patients with COPD and 18 healthy controls were included in this study.EBC was collected in all subjects with the experimental device.The concentrations of MPO in EBC were determined by enzyme-linked immunosorbent assay.Results The MPO level significantly increased in the COPD patients [（35.68 ± 0.32） μg/L],respectively compared with the healthy control group [（11.72± 0.34） μg/L] （P 〈0.05）.An inverse correlation was observed between MPO and spirometric parameters （FEV1 % pred,PEF）.Conclusions We concluded that the measurement of the EBC MPO level may be useful in screening and monitoring air inflammation and oxidative stress of patient with COPD,and the change of pathogenetic condition in COPD.The EBC MPO level in COPD patient could reflect the change of lung function and severity of disease to some extent.